Construct: ORF TRCN0000468929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016859.1_s317c1
- Derived from:
- ccsbBroadEn_08887
- DNA Barcode:
- GAGTAGAACAATCACTCTTTTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPBP1 (65056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_022913.4 | 99.9% | 100% | 174G>A |
| 2 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001127236.2 | 98.4% | 98.5% | 64_84del;195G>A |
| 3 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001331037.1 | 95.8% | 95.9% | 174G>A;478_537del |
| 4 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001127235.2 | 95.3% | 95.4% | 64_84del;195G>A;823_824ins45 |
| 5 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001203246.1 | 63.8% | 63.8% | 0_1ins513 |
| 6 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | NM_001122963.1 | 92.7% | 95.3% | (many diffs) |
| 7 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | NM_028487.4 | 88.9% | 91.4% | (many diffs) |
| 8 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_006517772.1 | 88.9% | 91.4% | (many diffs) |
| 9 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_006517775.3 | 68.1% | 66.3% | (many diffs) |
| 10 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_006517773.3 | 65.3% | 63.6% | (many diffs) |
| 11 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_017315613.1 | 59.1% | 61.3% | (many diffs) |
| 12 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_017315614.1 | 59.1% | 61.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1488
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcagcat gactttgctc cagcctggct taatttccct actccaccat 121 catcaacaaa gtcgtcattg aattttgaga agcattctga aaactttgca tggacagaga 181 atcgttatga tgtgaaccgt cgacgacaca actcttcaga tggctttgat tctgctattg 241 gacgtcctaa tggaggtaac tttggaagga aagaaaaaaa tggatggcgt acacatggaa 301 gaaatggtac agaaaacata aatcatcgag gtggatacca tggtggaagt tcccgttctc 361 gtagcagtat tttccatgca ggaaaaagcc aaggactaca tgaaaacaac atacctgaca 421 atgaaaccgg gaggaaagaa gacaagagag aacgcaaaca gtttgaagct gaggattttc 481 cgtctttaaa tcctgagtat gagagagaac caaatcacaa taagtcttta gctgcaggtg 541 tgtgggaata tcctccgaat cctaaatcta gagctccaag gatgctggtc attaagaaag 601 gtaatacaaa agacttacag ctatctggat tcccagtagt aggaaatctt ccgtcacagc 661 cagttaagaa tggaactggt ccaagtgttt ataaaggttt agtccctaaa cctgctgctc 721 cacctacaaa acctacacaa tggaaaagcc aaacaaaaga aaataaagtt ggaacttctt 781 tccctcatga gtccacattt ggcgttggca actttaatgc ttttaaatca actgccaaga 841 actttagtcc atctacaaat tcagtgaaag agtgtaatcg ctcaaattcc tcttctcctg 901 ttgacaaact taatcagcag cctcgtctaa ccaaactgac acgaatgcgc actgataaga 961 agagtgaatt tttgaaagca ttgaaaagag acagagtaga agaggaacat gaagatgaaa 1021 gccgtgctgg cTCAGAGAAG GATGACGACT CATTTAATTT ACATAACAGC AATAGTACTC 1081 ACCAAGAAAG GGATATAAAC CGAAACTTCG ATGAAAATGA AATTCCTCAA GAGAATGGCA 1141 ATGCCTCAGT GATTTCCCAG CAGATCATTC GGTCTTCAAC CTTCCCACAA ACTGATGTTC 1201 TTTCAAGTTC ACTTGAGGCA GAACACAGAT TGTTAAAGGA AATGGGCTGG CAGGAAGACA 1261 GTGAAAATGA TGAAACATGT GCTCCCTTAA CTGAGGATGA AATGAGAGAA TTCCAAGTTA 1321 TTAGTGAACA GTTACAGAAG AATGGTCTGA GAAAAAATGG TATTTTGAAA AATGGCTTGA 1381 TCTGTGACTT CAAGTTTGGA CCGTGGAAGA ACAGCACTTT CAAACCCACA ACTGAGAATG 1441 ATGACACAGA GACAAGTAGC AGTGATACAT CAGATGACGA CGATGTGTTG CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAGAGTAGA ACAATCACTC TTTTACAACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt