Construct: ORF TRCN0000468938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018001.1_s317c1
- Derived from:
- ccsbBroadEn_15915
- DNA Barcode:
- AGGTTCCTGCTAGTATCATAGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPN (55839)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55839 | CENPN | centromere protein N | NM_018455.5 | 99.8% | 99.5% | 252A>T |
| 2 | human | 55839 | CENPN | centromere protein N | XM_017023456.2 | 99.8% | 99.5% | 252A>T |
| 3 | human | 55839 | CENPN | centromere protein N | NM_001270474.1 | 63.2% | 59.3% | (many diffs) |
| 4 | human | 55839 | CENPN | centromere protein N | NM_001100624.2 | 57.8% | 51.8% | (many diffs) |
| 5 | human | 55839 | CENPN | centromere protein N | XM_006721236.4 | 57.8% | 51.8% | (many diffs) |
| 6 | human | 55839 | CENPN | centromere protein N | NM_001100625.2 | 55.6% | 49.8% | (many diffs) |
| 7 | human | 55839 | CENPN | centromere protein N | NM_001270473.1 | 52.1% | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgagactgtt gctgagttca tcaagaggac catcttgaaa atccccatga 121 atgaactgac aacaatcctg aaggcctggg attttttgtc tgaaaatcaa ctgcagactg 181 taaatttccg acagagaaag gaatctgtag ttcagcactt gatccatCTG TGTGAGGAAA 241 AGCGTGCAAG TATCAGTGAT GCTGCCCTGT TAGACATCAT TTATATGCAA TTTCATCAGC 301 ACCAGAAAGT TTGGGATGTT TTTCAGATGA GTAAAGGACC AGGTGAAGAT GTTGACCTTT 361 TTGATATGAA ACAATTTAAA AATTCGTTCA AGAAAATTCT TCAGAGAGCA TTAAAAAATG 421 TGACAGTCAG CTTCAGAGAA ACTGAGGAGA ATGCAGTCTG GATTCGAATT GCCTGGGGAA 481 CACAGTACAC AAAGCCAAAC CAGTACAAAC CTACCTACGT GGTGTACTAC TCCCAGACTC 541 CGTACGCCTT CACGTCCTCC TCCATGCTGA GGCGCAATAC ACCGCTTCTG GGTCAGGAGT 601 TAGAAGCTAC TGGGAAAATC TACCTCCGAC AAGAGGAGAT CATTTTAGAT ATTACCGAAA 661 TGAAGAAAGC TTGCAATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGGTTCC TGCTAGTATC 841 ATAGCCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt