Construct: ORF TRCN0000468940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004683.1_s317c1
- Derived from:
- ccsbBroadEn_01638
- DNA Barcode:
- GCAGCTTGCAGGTTATGGCCTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAGLN (6876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468940
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6876 | TAGLN | transgelin | NM_001001522.2 | 100% | 100% | |
| 2 | human | 6876 | TAGLN | transgelin | NM_003186.5 | 100% | 100% | |
| 3 | mouse | 21345 | Tagln | transgelin | NM_011526.5 | 91% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caacaagggt ccttcctatg gcatgagccg cgaagtgcag tccaaaatcg 121 agaagaagta tgacgaggag ctggaggagc ggctggtgga gtggatcata gtgcagtgtg 181 gccctgatgt gggccgccca gaccgtgggc gcttgggctt ccaggtctgg ctgaagaatg 241 gcgtgattct gagcaagctg gtgaacagcc tgtacccTGA TGGCTCCAAG CCGGTGAAGG 301 TGCCCGAGAA CCCACCCTCC ATGGTCTTCA AGCAGATGGA GCAGGTGGCT CAGTTCCTGA 361 AGGCGGCTGA GGACTATGGG GTCATCAAGA CTGACATGTT CCAGACTGTT GACCTCTTTG 421 AAGGCAAAGA CATGGCAGCA GTGCAGAGGA CCCTGATGGC TTTGGGCAGC TTGGCAGTGA 481 CCAAGAATGA TGGGCACTAC CGTGGAGATC CCAACTGGTT TATGAAGAAA GCGCAGGAGC 541 ATAAGAGGGA ATTCACAGAG AGCCAGCTGC AGGAGGGAAA GCATGTCATT GGCCTTCAGA 601 TGGGCAGCAA CAGAGGGGCC TCCCAGGCCG GCATGACAGG CTACGGACGA CCTCGGCAGA 661 TCATCAGTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCAGCT TGCAGGTTAT GGCCTGCCAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt