Construct: ORF TRCN0000468954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010896.1_s317c1
- Derived from:
- ccsbBroadEn_05147
- DNA Barcode:
- AACCGTGGCGAAGCCTACCCTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNBD1 (168975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 168975 | CNBD1 | cyclic nucleotide binding d... | NM_173538.3 | 100% | 100% | |
| 2 | human | 168975 | CNBD1 | cyclic nucleotide binding d... | XM_017013149.1 | 93.8% | 93.5% | 1303_1314del;1318A>G;1321_1392del |
| 3 | human | 168975 | CNBD1 | cyclic nucleotide binding d... | XM_024447082.1 | 64.5% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1377
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgatgtct tctcttccag cagctatttt gtctcacatg acagctatta 121 acaatgtgcc tcctcctcca cttcacagta taccaaactt gaaaaagtct aagcacatta 181 attatggcca gttgaatgca ttatgccaca ttagaggaca acacagccgg agtatgagca 241 atatcttatc agctcacgat acatttatga agcaatatcc taaagtattc ctgcaccaaa 301 aacccagact tcctaaactt ttcaaacagg aggaacaaag ggaactcaat gaaggcaaag 361 aggaaagtca acatcaacaa cctgatgatt ctaacaatat agctgtccat gttcagagag 421 cacatggtgg ccatatttta tatagaccaa aaagagccac agagaaattt gaagaattcc 481 tagctatctt aaagaaattg cccattcaca ggacgccata tgaacacaaa actgtgtgga 541 agttcctgaa aacaattcca gatttaacct ttcagctaaa tgataagcat ctgaaaacac 601 ttagtaagac tgtcttttcc gaaacctggt tgaaaggcag cacagtggtt gcaaatgatg 661 gattttatgt aatactgaaa ggcctagctc gacctcaaac aaacgtgtat aaaaatctga 721 ttgaaggaag tgattcacca gactcgttca tatctcagag tttccacagc ttcatttgga 781 gtgaagaatt caaaaactct acacttgctg agatgtacct accttcatat gactcaatgc 841 ttagcaaatg gagtaccttt gggactctgg aagttatgcc tcagaatgaa tcggaaacac 901 agatgttctc ggtggtgaca gaagacgatt gtgaaattct taaaatccca gcaaagggat 961 atgcaaagat aaaggaggaa aaaataaaac ttGAAAATAT GCAAAAGTTG AAATTAATCC 1021 GTATGTGTCC TTATTATGAG GAATGGCCTA CTTTATCCAT ATATGAGCTA ATTGCACTCC 1081 TTAAATGGAA AAAATTTCCT CCAGGTCATG TGATAGTGGA AAGTGGAAAT ATAATTTCTT 1141 TTGTGGGTTA TATTAACTCT GGATGCTGTA ACATTTATAG AAGTATTATA GGATTTGTGA 1201 AACTACGATC AAATAAAGTG AAAAGATCTC AAAAACTTGT TTATATGGGG AAACTTAAGG 1261 AGAAGGAGTC CTTTGGTGAG ATTAGCGTCC TTCTTCAAGT TCCTTTCACG TGCACAATCA 1321 TTACCAAAAA AGAAGTTGAG ATGGCAATCA TTGAAGATAA GGACCTATTT GTGGCCTTGC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GAAACCGTGG CGAAGCCTAC CCTAACACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt