Construct: ORF TRCN0000468960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010385.1_s317c1
- Derived from:
- ccsbBroadEn_10257
- DNA Barcode:
- TCGGCCTTCATGGCTCTATACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA2P10 (80154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84559 | GOLGA2P2Y | GOLGA2 pseudogene 2 Y-linked | NR_001555.2 | 22.8% | (many diffs) | |
| 2 | human | 401634 | GOLGA2P3Y | GOLGA2 pseudogene 3 Y-linked | NR_002195.2 | 22.8% | (many diffs) | |
| 3 | human | 101929479 | LOC101929479 | golgin A2 pseudogene | NR_158179.1 | 16.8% | 1_350del;609_1532del | |
| 4 | human | 653061 | GOLGA8S | golgin A8 family member S | XM_024450031.1 | 16.5% | 11% | (many diffs) |
| 5 | human | 101929479 | LOC101929479 | golgin A2 pseudogene | NR_160936.1 | 12.2% | (many diffs) | |
| 6 | human | 653073 | GOLGA8J | golgin A8 family member J | XR_002957674.1 | 9.8% | (many diffs) | |
| 7 | human | 653720 | GOLGA8M | golgin A8 family member M | XM_011521952.3 | 9.1% | 5.7% | (many diffs) |
| 8 | human | 80154 | GOLGA2P10 | GOLGA2 pseudogene 10 | NR_026811.3 | 8.2% | (many diffs) | |
| 9 | human | 727751 | LOC727751 | golgin A2 pseudogene | NR_102748.1 | 8.1% | (many diffs) | |
| 10 | human | 102724135 | LOC102724135 | uncharacterized LOC102724135 | NR_158192.1 | 8.1% | (many diffs) | |
| 11 | human | 80154 | GOLGA2P10 | GOLGA2 pseudogene 10 | NR_103496.2 | 8% | (many diffs) | |
| 12 | human | 729668 | GOLGA2P6 | GOLGA2 pseudogene 6 | NR_120609.1 | 7.8% | (many diffs) | |
| 13 | human | 2801 | GOLGA2 | golgin A2 | XM_005251932.1 | 7.8% | 6.3% | (many diffs) |
| 14 | human | 2801 | GOLGA2 | golgin A2 | NM_001366246.1 | 7.7% | 6.2% | (many diffs) |
| 15 | human | 2801 | GOLGA2 | golgin A2 | NM_004486.5 | 7.7% | 6.2% | (many diffs) |
| 16 | human | 2801 | GOLGA2 | golgin A2 | NM_001366244.1 | 7.5% | 6% | (many diffs) |
| 17 | human | 388152 | GOLGA2P7 | GOLGA2 pseudogene 7 | NR_027001.1 | 7.2% | (many diffs) | |
| 18 | human | 80154 | GOLGA2P10 | GOLGA2 pseudogene 10 | NR_033936.3 | 7.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 220
- ORF end:
- 478
- ORF length:
- 258
- Sequence:
-
1 AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG 61 CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA 121 TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT 181 AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCGAACAT CCCAGGGGAC 241 CTGGAGAGCC GGGAGGCCAT GGTGGCATTT TTCAACTCCG CTGGAGCCAG TGCCCAGGAG 301 GAACAAAGGG TGTGCTGCCA GCCCCTGGCT CACCCAGTGG CCTCGTCCCA GAAGAAGCCA 361 GAGGTAGCGG CCCCAGCCCC AGAGAGTGGG GGTGAGTCTG TGTTTGGGGA GACCCACCGG 421 GCCCTGCAGG GGGCCATGGA GAAGCTGCAG CGACTTTATG GAAGGAGAAG GTGGACCTGC 481 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 541 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 601 TATATATCTT GTGGAAAGGA CGATCGGCCT TCATGGCTCT ATACCCTACG CGTTAAGTCg 661 acaatcaacc tctggattac aaaatttgtg aaagatt