Construct: ORF TRCN0000468965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007016.1_s317c1
- Derived from:
- ccsbBroadEn_00099
- DNA Barcode:
- ATTGGGCATGGAAGTTAGTTAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARG2 (384)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 384 | ARG2 | arginase 2 | NM_001172.4 | 100% | 100% | |
| 2 | mouse | 11847 | Arg2 | arginase type II | NM_009705.3 | 85.9% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctaaggggc agcctctcgc gtctcctcca gacgcgagtg cattccatcc 121 tgaagaaatc cgtccactcc gtggctgtga taggagcccc gttctcacaa gggcagaaaa 181 gaaaaggagt ggagcatggt cccgctgcca taagagaagc tggcttgatg aaaaggctct 241 ccagtttggg ctgccaccta aaagactttg gagatttgag ttttactcca gtccccaaag 301 atgatctcta caacaacctg atagtgaatc cacgctcagt gggtcttgcc aaccaggaac 361 tggctgaggt ggttagcaga gctgtgtcag atggctacag ctgtgtcaca ctgggaggag 421 accacagcct ggcaatcggt accattagtg gccatgcccg acactgccca gacctttgtg 481 ttgtctgggt tgatgcccat gctgacatca acacacccct taccacttca tcaggaaatc 541 tccatggaca gccagtttca tttctcctca gagaactaca ggataaggta ccacaactcc 601 caggattttc ctggatcaaa ccttgtatct cttctgcaag tattgtgtat attggTCTGA 661 GAGACGTGGA CCCTCCTGAA CATTTTATTT TAAAGAACTA TGATATCCAG TATTTTTCCA 721 TGAGAGATAT TGATCGACTT GGTATCCAGA AGGTCATGGA ACGAACATTT GATCTGCTGA 781 TTGGCAAGAG ACAAAGACCA ATCCATTTGA GTTTTGATAT TGATGCATTT GACCCTACAC 841 TGGCTCCAGC CACAGGAACT CCTGTTGTCG GGGGACTAAC CTATCGAGAA GGCATGTATA 901 TTGCTGAGGA AATACACAAT ACAGGGTTGC TATCAGCACT GGATCTTGTT GAAGTCAATC 961 CTCAGTTGGC CACCTCAGAG GAAGAGGCGA AGACTACAGC TAACCTGGCA GTAGATGTGA 1021 TTGCTTCAAG CTTTGGTCAG ACAAGAGAAG GAGGGCATAT TGTCTATGAC CAACTTCCTA 1081 CTCCCAGTTC ACCAGATGAA TCAGAAAATC AAGCACGTGT GAGAATTTAC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAATTGGGC ATGGAAGTTA GTTAGAAACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt