Construct: ORF TRCN0000468976
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005464.1_s317c1
- Derived from:
- ccsbBroadEn_08178
- DNA Barcode:
- AGTCTGCCGCCATTTATGGACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT9 (50614)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468976
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50614 | GALNT9 | polypeptide N-acetylgalacto... | NM_021808.3 | 99.5% | 100% | 78T>C;420T>C;423A>C |
| 2 | human | 50614 | GALNT9 | polypeptide N-acetylgalacto... | NM_001122636.2 | 39.1% | 39.3% | (many diffs) |
| 3 | mouse | 231605 | Galnt9 | UDP-N-acetyl-alpha-D-galact... | NM_001122639.2 | 86.3% | 95.7% | (many diffs) |
| 4 | mouse | 231605 | Galnt9 | UDP-N-acetyl-alpha-D-galact... | NR_104449.1 | 38.4% | (many diffs) | |
| 5 | mouse | 231605 | Galnt9 | UDP-N-acetyl-alpha-D-galact... | NM_198306.3 | 33.8% | 37.5% | (many diffs) |
| 6 | mouse | 231605 | Galnt9 | UDP-N-acetyl-alpha-D-galact... | XR_389273.2 | 24.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 780
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggtgctg ccctgctccc gcgtggccca catcgagcgc accaggaagc 121 cctacaacaa cgacattgac tactacgcca agcgcaacgc cctgcgcgcc gccgaggtgt 181 ggatggatga cttcaagtcc cacgtgtaca tggcctggaa catccccatg tcgaacccag 241 gggtggactt cggggacgtg tctgagaggc tggccctgcg tcagaggctg aagtgtcgca 301 gcttcaagtg gtacctggag aacgtgtacc cggagatgag ggtctacaac aacaccctca 361 cgtacggaga ggtgagaaac agcaaagcca gtgccTACTG TCTGGACCAG GGAGCGGAGG 421 ACGGCGACCG GGCGATCCTC TACCCCTGCC ACGGGATGTC CTCCCAGCTG GTGCGGTACA 481 GCGCTGACGG CCTGCTGCAG CTGGGGCCTC TGGGCTCCAC AGCCTTCTTG CCTGACTCCA 541 AGTGTCTGGT GGATGACGGC ACGGGCCGCA TGCCCACCCT GAAGAAGTGT GAGGATGTGG 601 CGCGGCCAAC ACAGCGGCTG TGGGACTTCA CCCAGAGTGG CCCCATTGTG AGCCGGGCCA 661 CGGGCCGCTG CCTGGAGGTG GAGATGTCCA AAGATGCCAA CTTTGGGCTC CGGCTGGTGG 721 TACAGAGGTG CTCGGGGCAG AAGTGGATGA TCAGAAACTG GATCAAACAC GCACGGCACT 781 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGAAGTCT GCCGCCATTT ATGGACTACA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt