Construct: ORF TRCN0000468983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008799.1_s317c1
- Derived from:
- ccsbBroadEn_13500
- DNA Barcode:
- ACCAGTTCCAATGTAGGCCAGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf40 (283461)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283461 | C12orf40 | chromosome 12 open reading ... | XM_005268806.3 | 100% | 100% | |
| 2 | human | 283461 | C12orf40 | chromosome 12 open reading ... | XM_011538231.2 | 100% | 100% | |
| 3 | human | 283461 | C12orf40 | chromosome 12 open reading ... | NM_001031748.4 | 88.1% | 88.1% | 1_231del |
| 4 | human | 283461 | C12orf40 | chromosome 12 open reading ... | NM_001319247.2 | 61% | 60.4% | (many diffs) |
| 5 | human | 283461 | C12orf40 | chromosome 12 open reading ... | NR_135051.2 | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tagagacata aaaatgcccc taagaaagca taatttagaa cttacaatgt 121 cgcctcactg tgtaccttct aaactctgcc ttgatgatac agaaaccaat gtaaactgtc 181 aaagactaag tagcaaggaa gatcttggcc cagtccagtc acaaggcatg gactcatata 241 gtatgcttca ccctcagttc agcaaaatag agaactgcag tttcactcca tcatcttttt 301 cagtggagtt accttctaac agacatattt caaaactaaa tttcacatct ggaatagcac 361 ctactcctca gaaacttgca tatgaaaaaa agcagaatga ccagcgcagt actgttaact 421 gttctgattc cttgctttcc aaattaaata aaagtcaaga tgttttcagt ccatcacata 481 aaactacacg atttgggaca ttatttgaaa gattaaacag tctaggaaat aggaatttac 541 ttactaaaag ccctgctgta attatggatg aagattgtag aagcacggat gaaataagac 601 agtcagacta cattactgaa aaacactcaa tacagcatat ttgggggaaa aatggaaagg 661 aagtttcaaa ttttcttgaa gatgtgaacc agtctactcc taaccttcta tcagagaatt 721 gtgactcttt tgttagtcaa aatatgatca atgtattaaa catagatgag caaaggataa 781 agaaaacatt taacaagtgt gattatgata gtatgggaga tacttgtgta gtcactagtt 841 ctgataaaaa ccatgtcact gacagatgca ttagaaacat ttttacagtt ccagagttga 901 cttttagtaa ttcgactttg aataaaacaa gttatccaga aaaatgtcag ccaaacaaga 961 agtatcagag agagtataac aaaaatgaaa gaaatgatct tagtacatct tttgagaacg 1021 attactaccc aagcagctct gaaagaaaag aaaaatttga aaatgattac caagagaaga 1081 caccacagaa aagtattcag aaatatccag caaactctat gggaaatata ccttcggaag 1141 aattgcactc taagcagtca tgggactttg gacttgatga gattctgatg gaagaaggag 1201 gaatatattc tttaaaaagc aagcgaatat ctactaagaa aatctctctt gattctgcac 1261 agagtagtcg gtctactagt tactctccaa gaccaacaga cagctgtttc agttctagtt 1321 cagatttgcc atcTGAAGAT GAAGATCAAA TATCACAGCA AATTGAAGAT TCAAATAGGA 1381 TGACCATCAA AACCAAGGAA AAAATGAATA ATTTTTATGT AGAAAGGATG GCAAAACTTT 1441 CAGGTGACAG GATTGTTAAA AACGATGACA AAATTCACAA ACAGAATGAG AATTTCTACC 1501 AGTTCTCAGT GAAAAATAAT ACAGATCAGT TTCCACAGTT GCAGTGCAAT TCAGCCCACA 1561 TTTTGCAAAA CAAAACCAAT GATAACTGCG TTCTGCAGGC TGCAAGGTGT GATGCAGGGA 1621 TACAAACAGA GAGTGAATCT GTAATGGAGG AAAAATTAGA TGTTGCCATA CAGTGTGATC 1681 TAATTTCAAA ATGTACATGT AGAAGTGATG TTTCTCTTTG CAACCTTGAA AGGTGCAGTG 1741 GAAATATTAA GGCAGATACC ACTGGAGGGC AGGAAATCCA TAAGAACAAC TACCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGAACCA GTTCCAATGT AGGCCAGACG ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt