Construct: ORF TRCN0000469010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017772.1_s317c1
- Derived from:
- ccsbBroadEn_00701
- DNA Barcode:
- TTTTTAGACCATACGTGGGATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTA1 (2938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469010
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2938 | GSTA1 | glutathione S-transferase a... | NM_145740.5 | 100% | 100% | |
| 2 | human | 2939 | GSTA2 | glutathione S-transferase a... | NM_000846.5 | 96.6% | 95% | (many diffs) |
| 3 | human | 2939 | GSTA2 | glutathione S-transferase a... | XM_011514532.3 | 96.6% | 95% | (many diffs) |
| 4 | human | 221357 | GSTA5 | glutathione S-transferase a... | NM_153699.1 | 93.5% | 90% | (many diffs) |
| 5 | human | 2940 | GSTA3 | glutathione S-transferase a... | NM_000847.5 | 92.4% | 90.5% | (many diffs) |
| 6 | human | 2938 | GSTA1 | glutathione S-transferase a... | XM_005249034.4 | 83.3% | 80.6% | (many diffs) |
| 7 | human | 2940 | GSTA3 | glutathione S-transferase a... | NM_001363542.2 | 72% | 71.1% | (many diffs) |
| 8 | human | 2940 | GSTA3 | glutathione S-transferase a... | XM_006715072.4 | 72% | 71.1% | (many diffs) |
| 9 | human | 2940 | GSTA3 | glutathione S-transferase a... | XM_024446414.1 | 72% | 71.1% | (many diffs) |
| 10 | human | 2938 | GSTA1 | glutathione S-transferase a... | NM_001319059.2 | 58.1% | 58.1% | 0_1ins279 |
| 11 | human | 2940 | GSTA3 | glutathione S-transferase a... | XM_011514533.2 | 53.4% | 51.8% | (many diffs) |
| 12 | human | 2940 | GSTA3 | glutathione S-transferase a... | XR_926166.2 | 48% | (many diffs) | |
| 13 | human | 2940 | GSTA3 | glutathione S-transferase a... | XR_926167.2 | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaagccc aagctccact acttcaatgc acggggcaga atggagtcca 121 cccggtggct cctggctgca gctggagtag agtttgaaga gaaatttata aaatctgcag 181 aagatttgga caagttaaga aatgatggat atttgatgtt ccagcaagtg ccaatggttg 241 agattgatgg gatgaagctg gtgcagacca gagccattct caactacatt gccagcaaat 301 acaacctcta tgggaaagac ataaaggaga gagccctgat tgatatgtat atagaaggta 361 tagcagattt gggtgaaatg atcctccTTC TGCCCGTATG TCCACCTGAG GAAAAAGATG 421 CCAAGCTTGC CTTGATCAAA GAGAAAATAA AAAATCGCTA CTTCCCTGCC TTTGAAAAAG 481 TCTTAAAGAG CCATGGACAA GACTACCTTG TTGGCAACAA GCTGAGCCGG GCTGACATTC 541 ATCTGGTGGA ACTTCTCTAC TACGTCGAGG AGCTTGACTC CAGTCTTATC TCCAGCTTCC 601 CTCTGCTGAA GGCCCTGAAA ACCAGAATCA GCAACCTGCC CACAGTGAAG AAGTTTCTAC 661 AGCCTGGCAG CCCAAGGAAG CCTCCCATGG ATGAGAAATC TTTAGAAGAA GCAAGGAAGA 721 TTTTCAGGTT TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT TTAGACCATA CGTGGGATTT 901 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t