Construct: ORF TRCN0000469011

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014314.1_s317c1
Derived from:
ccsbBroadEn_05790
DNA Barcode:
ATTAGTTTCGTGTCTTGCTCTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AGA (175)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469011
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 175 AGA aspartylglucosaminidase NM_000027.4 99.9% 99.7% 446C>G
2 human 175 AGA aspartylglucosaminidase NM_001171988.1 97% 96.8% 446C>G;675_676ins30
3 human 175 AGA aspartylglucosaminidase XR_001741155.2 49% (many diffs)
4 human 175 AGA aspartylglucosaminidase NR_033655.1 45.5% (many diffs)
5 mouse 11593 Aga aspartylglucosaminidase NM_001005847.2 84.2% 82.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1104
ORF length:
1038
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcggaagtcg aacttgcctg tgcttctcgt gccgtttctg ctctgccagg
121ccctagtgcg ctgctccagc cctctgcccc tggtcgtcaa cacttggccc tttaagaatg
181caaccgaagc agcgtggagg gcattagcat ctggaggctc tgccctggat gcagtggaga
241gcggctgtgc catgtgtgag agagagcagt gtgacggctc tgtaggcttt ggaggaagtc
301ctgatgaact tggagaaacc acactagatg ccatgatcat ggatggcact actatggatg
361taggagcagt aggagatctc agacgaatta aaaatgctat tggtgtggca cggaaagtac
421tggaacatac aacacacaca cttttagtag gagagtcagc caccacattt gctcaaagta
481tggggtttat caatgaagac ttatctacca gtgcttctca agctcttcat tcagattggc
541ttgctcggaa ttgccagcca aattattgga ggaatgttat accagatccc tcaaaatact
601gcggacccta caaaccacct ggtatcttaa agcaggatat tcctatccat aaagaaacag
661aagatgatcg tggtcatgac actattggca tggttgtaat ccataagaca ggacatattg
721ctgctggtac atctacaaat ggtataaaat tcaaaataca tggccgtgta ggagactcac
781caatacctGG AGCTGGAGCC TATGCTGACG ATACTGCAGG GGCAGCCGCA GCCACTGGGA
841ATGGTGATAT ATTGATGCGC TTCCTGCCAA GCTACCAAGC TGTAGAATAC ATGAGAAGAG
901GAGAAGATCC AACCATAGCT TGCCAAAAAG TGATTTCAAG AATCCAGAAG CATTTTCCAG
961AATTCTTTGG GGCTGTTATA TGTGCCAATG TGACTGGAAG TTACGGTGCT GCTTGCAATA
1021AACTTTCAAC ATTTACTCAG TTTAGTTTCA TGGTTTATAA TTCCGAAAAA AATCAGCCAA
1081CTGAGGAAAA AGTGGACTGC ATCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1141AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1201TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTAGTTTCGT
1261GTCTTGCTCT CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1321att

Download FASTA (ORF) (Full)

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