Construct: ORF TRCN0000469011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014314.1_s317c1
- Derived from:
- ccsbBroadEn_05790
- DNA Barcode:
- ATTAGTTTCGTGTCTTGCTCTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGA (175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 175 | AGA | aspartylglucosaminidase | NM_000027.4 | 99.9% | 99.7% | 446C>G |
| 2 | human | 175 | AGA | aspartylglucosaminidase | NM_001171988.1 | 97% | 96.8% | 446C>G;675_676ins30 |
| 3 | human | 175 | AGA | aspartylglucosaminidase | XR_001741155.2 | 49% | (many diffs) | |
| 4 | human | 175 | AGA | aspartylglucosaminidase | NR_033655.1 | 45.5% | (many diffs) | |
| 5 | mouse | 11593 | Aga | aspartylglucosaminidase | NM_001005847.2 | 84.2% | 82.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1104
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggaagtcg aacttgcctg tgcttctcgt gccgtttctg ctctgccagg 121 ccctagtgcg ctgctccagc cctctgcccc tggtcgtcaa cacttggccc tttaagaatg 181 caaccgaagc agcgtggagg gcattagcat ctggaggctc tgccctggat gcagtggaga 241 gcggctgtgc catgtgtgag agagagcagt gtgacggctc tgtaggcttt ggaggaagtc 301 ctgatgaact tggagaaacc acactagatg ccatgatcat ggatggcact actatggatg 361 taggagcagt aggagatctc agacgaatta aaaatgctat tggtgtggca cggaaagtac 421 tggaacatac aacacacaca cttttagtag gagagtcagc caccacattt gctcaaagta 481 tggggtttat caatgaagac ttatctacca gtgcttctca agctcttcat tcagattggc 541 ttgctcggaa ttgccagcca aattattgga ggaatgttat accagatccc tcaaaatact 601 gcggacccta caaaccacct ggtatcttaa agcaggatat tcctatccat aaagaaacag 661 aagatgatcg tggtcatgac actattggca tggttgtaat ccataagaca ggacatattg 721 ctgctggtac atctacaaat ggtataaaat tcaaaataca tggccgtgta ggagactcac 781 caatacctGG AGCTGGAGCC TATGCTGACG ATACTGCAGG GGCAGCCGCA GCCACTGGGA 841 ATGGTGATAT ATTGATGCGC TTCCTGCCAA GCTACCAAGC TGTAGAATAC ATGAGAAGAG 901 GAGAAGATCC AACCATAGCT TGCCAAAAAG TGATTTCAAG AATCCAGAAG CATTTTCCAG 961 AATTCTTTGG GGCTGTTATA TGTGCCAATG TGACTGGAAG TTACGGTGCT GCTTGCAATA 1021 AACTTTCAAC ATTTACTCAG TTTAGTTTCA TGGTTTATAA TTCCGAAAAA AATCAGCCAA 1081 CTGAGGAAAA AGTGGACTGC ATCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTAGTTTCGT 1261 GTCTTGCTCT CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att