Construct: ORF TRCN0000469021
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013978.1_s317c1
- Derived from:
- ccsbBroadEn_07879
- DNA Barcode:
- ATTCTACCGAGCATCACCAGCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COTL1 (23406)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469021
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23406 | COTL1 | coactosin like F-actin bind... | NM_021149.5 | 99.7% | 99.2% | 13A>T |
| 2 | mouse | 72042 | Cotl1 | coactosin-like 1 (Dictyoste... | NM_028071.3 | 87.5% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tttgcatggc caccaagttc gacaaagagg cttgccgggc ggcgtacaac cTGGTGCGCG 121 ACGACGGCTC GGCCGTCATC TGGGTGACTT TTAAATATGA CGGCTCCACC ATCGTCCCCG 181 GCGAGCAGGG AGCGGAGTAC CAGCACTTCA TCCAGCAGTG CACAGATGAC GTCCGGTTGT 241 TTGCCTTCGT GCGCTTCACC ACCGGGGATG CCATGAGCAA GAGGTCCAAG TTTGCCCTCA 301 TCACGTGGAT CGGTGAGAAC GTCAGCGGGC TGCAGCGCGC CAAAACCGGG ACGGACAAGA 361 CCCTGGTGAA GGAGGTCGTA CAGAATTTCG CTAAGGAGTT TGTGATCAGT GATCGGAAGG 421 AGCTGGAGGA AGATTTCATC AAGAGCGAGC TGAAGAAGGC GGGGGGAGCC AATTACGACG 481 CCCAGACGGA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATT CTACCGAGCA TCACCAGCTC 661 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t