Construct: ORF TRCN0000469037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003390.1_s317c1
- Derived from:
- ccsbBroadEn_14298
- DNA Barcode:
- GAATGTTTCTGAAAGGGAGACTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FRMD8 (83786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83786 | FRMD8 | FERM domain containing 8 | NM_031904.5 | 99.8% | 2.3% | 10delA;339C>T |
| 2 | human | 83786 | FRMD8 | FERM domain containing 8 | NM_001300833.3 | 92.6% | 2.5% | 10delA;250_251ins102 |
| 3 | human | 83786 | FRMD8 | FERM domain containing 8 | NM_001300832.3 | 87.7% | 2.2% | 10delA;84_85ins168;171C>T |
| 4 | human | 83957 | FRMD8P1 | FERM domain containing 8 ps... | NR_033742.1 | 73.7% | (many diffs) | |
| 5 | mouse | 67457 | Frmd8 | FERM domain containing 8 | NM_026169.4 | 84.3% | 2.3% | (many diffs) |
| 6 | mouse | 67457 | Frmd8 | FERM domain containing 8 | XM_006531828.2 | 84.3% | 2.3% | (many diffs) |
| 7 | mouse | 67457 | Frmd8 | FERM domain containing 8 | XM_006531829.3 | 66.5% | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 171
- ORF length:
- 105
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgggcagaag gcagtgccgg gcagcccggc cccgctgagc gatcccaccg 121 aagcagcgtg tcctccgtgg gagcccgagc ggctgacgtg ctggtatacc tagcggatga 181 cacggtggtg cccctggctg tggagaacct gccctcgctc agtgcccatg agctgcaccg 241 cgctgtccgc gaggtcctgc agcttccaga catcgccctg gatgtcttcg cgctctggct 301 ggtctcccct ctgctggagg tgcagctgaa acccaagcac cagccctaca agctgggacg 361 ccagtggccg gagctgctgc tgcgcttcac cagtgcccca gatgatgacg tggccatgga 421 tgagcctttc ctgcagttcc gaaggaacgt gttcttccca aagcggcggg agctccagat 481 ccatgacgag gaggtcctgc ggctgctcta tgaggaggcc aagggcaacg tgctggctgc 541 acggtacccg tgcgacgtgg aggactgcga ggctctgggc gccctggtgt gccgcgtgca 601 gcttgggccc taccagcccg gccggccggc agcctgcgac ctgagggaga agctggactc 661 cttcctccct gcccacctct gtaagcgggg ccagagtctc tttgctgccc tccggggccg 721 tggggccagg gccgggccgg gcgagcaggg cctgctgaac gcctaccgcc aggtgcagga 781 ggtcagcagc gacggcgggt gcgaggccgc cctgggcacc cactaccgcg cctatctcct 841 caagtgccac gagctgccgt tttatgggtg tgccttcttc cacggtgagg ttgacaagcc 901 ggcccaaggc tttttgcacc ggggtgggcg caagccagtt tctgtggcca tcagtctgga 961 aggcgtgcac gtcatcgata gcagagagaa gcatgtcctg ctgggcctgc gcttccagga 1021 gctgtcgtgg gaccacaccT CCCCCGAGGA GGAGGAGCCC ATCTTGTGGC TGGAGTTCGA 1081 CGGGGACAGC GAGGGCACAC CTGTCAACAA GCTCCTCAAG ATCTACTCCA AGCAGGCCGA 1141 ACTGATGAGC AGTCTCATTG AGTACTGCAT CGAACTGAGC CAGGCGGCGG AGCCCGCAGG 1201 CCCCCAGGAC AGTGCGACTG GCTCGCCCTC GGACCCCAGC TCCTCACTGG CTCCTGTTCA 1261 GCGCCCCAAG CTGCGGAGGC AGGGCAGTGT GGTGTCCAGC CGGATCCAGC ATCTCTCCAC 1321 CATCGACTAC GTGGAGGACG GCAAGGGGAT CAGGCGAGTG AAGCCGAAGC GCACCACATC 1381 CTTCTTCAGC CGGCAGCTGT CCTTGGGCCA GGGGAGCTAC ACCGTGGTGC AGCCCGGCGA 1441 CAGCCTGGAG CAGGGCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 1501 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGCCCGT AACTTGAAAG 1561 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAATGTTT CTGAAAGGGA 1621 GACTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt