Construct: ORF TRCN0000469042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003481.1_s317c1
- Derived from:
- ccsbBroadEn_13926
- DNA Barcode:
- ACACTTGCTCAATAGCTTAGAATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP2R5D (5528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5528 | PPP2R5D | protein phosphatase 2 regul... | NM_006245.4 | 99.8% | .9% | 5C>G;17_18insT;1796A>C |
| 2 | human | 5528 | PPP2R5D | protein phosphatase 2 regul... | NM_180976.3 | 94.5% | 1% | (many diffs) |
| 3 | human | 5528 | PPP2R5D | protein phosphatase 2 regul... | NM_180977.3 | 81.4% | 1.1% | (many diffs) |
| 4 | human | 5528 | PPP2R5D | protein phosphatase 2 regul... | NM_001270476.2 | 74.8% | 1.1% | 0_1ins454;1343A>C |
| 5 | mouse | 21770 | Ppp2r5d | protein phosphatase 2, regu... | NM_009358.3 | 89.3% | 1.6% | (many diffs) |
| 6 | mouse | 21770 | Ppp2r5d | protein phosphatase 2, regu... | XM_006524029.2 | 89.2% | 1.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 129
- ORF length:
- 63
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ctataaactg aataaaggag aaggagcccc ccaaggttgc caaatgcaca 121 gccaagccta gcagctcggg caaggatggt ggaggcgaga acactgagga ggcccagccg 181 cagccccagc cccagcccca gccccaagcc cagtctcagc caccgtcatc caacaagcgt 241 cccagcaata gcacgccgcc ccccacgcag ctcagcaaaa tcaagtactc aggggggccc 301 cagattgtca agaaggagcg acggcaaagc tcctcccgct tcaacctcag caagaatcgg 361 gagctgcaga agcttcctgc cctgaaagat tcgccaaccc aggagcggga ggagctgttt 421 atccagaagc tacgccagtg ctgtgtcctc tttgacttcg tgtcagaccc actcagtgac 481 ctcaaattca aggaggtgaa gcgggcagga ctcaacgaga tggtggagta catcacccat 541 agccgtgatg ttgtcactga ggccatttac cctgaggctg tcaccatgtt ttcagtgaac 601 ctcttccgga cgctgccacc ttcatcgaat cccacagggg ctgagtttga cccagaggaa 661 gatgagccca ccctggaagc tgcttggcca catctccagc tcgtgtatga gttcttctta 721 cgtttccttg agtctcctga tttccagcca aacatagcca agaagtacat cgaccagaag 781 tttgtacttg ctctcctaga cctatttgac agtgaggatc ctcgagagcg ggacttcctc 841 aagaccattt tgcatcgcat ctatggcaag tttttggggc tccgggctta tatccgtagg 901 cagatcaacc acatcttcta caggttcatc tacgagacgg agcatcacaa cgggattgct 961 gagctcctgg agatcctggg cagcatcatc aatggctttg ccctgcccct taaagaagag 1021 cacaagatgt tcctcatccg tgtcctactt ccccttcaca aggtcaagtc cctgagtgtc 1081 taccaccctc agctggcata ctgtgtggta caattcctgg agaaggagag cagtctgact 1141 gagccggtaa ttgtgggact tctcaagttt tggcccaaga cccacagccc caaggaggtg 1201 atgttcttga atgagctgga ggagattctg gacgtcattg aaccttctga gttcagcaaa 1261 gtgatggaac ccctcttccg ccagctggcc aagtgtgtct ctagccccca tttccaggtg 1321 gcagagcgtg ctctctatta ctggaacaat gagtacatca tgagcctgat aagtgacaat 1381 gctgcccgag tcctccccat catgttccct gcactctaca ggaactccaa gagccactgg 1441 aacaagacaa tCCATGGACT GATCTATAAT GCCCTGAAGT TGTTTATGGA AATGAATCAG 1501 AAGCTGTTTG ATGACTGCAC ACAACAATAC AAGGCAGAGA AGCAGAAGGG CCGGTTCCGA 1561 ATGAAGGAAA GGGAAGAGAT GTGGCAAAAA ATCGAGGAGC TGGCCCGGCT TAATCCCCAG 1621 TATCCCATGT TCCGAGCCCC TCCACCACTG CCCCCTGTGT ACTCGATGGA GACAGAGACC 1681 CCCACAGCTG AGGACATCCA GCTTCTGAAG AGGACTGTGG AGACTGAGGC TGTTCAGATG 1741 CTAAAAGACA TCAAGAAGGA GAAAGTGCTG CTGCGGAGGA AGTCGGAGCT GCCCCAGGAC 1801 GTGTACACCA TCAAGGCACT GGAGGCGCAC AAGCGGGCGG AAGAGTTCCT AACTGCCAGC 1861 CCGGAGGCTC TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 1921 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT 1981 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC ACTTGCTCAA TAGCTTAGAA 2041 TAACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt