Transcript: Mouse NM_009358.3

Mus musculus protein phosphatase 2, regulatory subunit B', delta (Ppp2r5d), mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Ppp2r5d (21770)
Length:
2926
CDS:
144..1928

Additional Resources:

NCBI RefSeq record:
NM_009358.3
NBCI Gene record:
Ppp2r5d (21770)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009358.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306033 AGACCCTAGAGAGCGGGATTT pLKO_005 869 CDS 100% 10.800 15.120 N Ppp2r5d n/a
2 TRCN0000080667 CATCGCATCTATGGCAAGTTT pLKO.1 906 CDS 100% 5.625 4.500 N Ppp2r5d n/a
3 TRCN0000306102 TCATGTTTCCTGCACTCTATA pLKO_005 1453 CDS 100% 13.200 9.240 N Ppp2r5d n/a
4 TRCN0000080664 GCCCTCTACTACTGGAACAAT pLKO.1 1383 CDS 100% 5.625 3.938 N Ppp2r5d n/a
5 TRCN0000353991 GCCCTCTACTACTGGAACAAT pLKO_005 1383 CDS 100% 5.625 3.938 N Ppp2r5d n/a
6 TRCN0000080665 CCAGCCAAATATAGCCAAGAA pLKO.1 797 CDS 100% 4.950 3.465 N Ppp2r5d n/a
7 TRCN0000080666 GCATCGCATCTATGGCAAGTT pLKO.1 905 CDS 100% 4.950 3.465 N Ppp2r5d n/a
8 TRCN0000325682 GCATCGCATCTATGGCAAGTT pLKO_005 905 CDS 100% 4.950 3.465 N Ppp2r5d n/a
9 TRCN0000080663 CCTGAAACTGTTCTGAGAAAT pLKO.1 2211 3UTR 100% 13.200 7.920 N Ppp2r5d n/a
10 TRCN0000325753 CCTGAAACTGTTCTGAGAAAT pLKO_005 2211 3UTR 100% 13.200 7.920 N Ppp2r5d n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009358.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13926 pDONR223 100% 89.3% 1.6% None (many diffs) n/a
2 ccsbBroad304_13926 pLX_304 0% 89.3% 1.6% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000469042 ACACTTGCTCAATAGCTTAGAATA pLX_317 23.6% 89.3% 1.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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