Construct: ORF TRCN0000469046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015165.1_s317c1
- Derived from:
- ccsbBroadEn_02452
- DNA Barcode:
- CCACTGTAACTGATTCTGACATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXF1 (10482)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469046
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10482 | NXF1 | nuclear RNA export factor 1 | NM_006362.5 | 100% | 100% | |
| 2 | human | 10482 | NXF1 | nuclear RNA export factor 1 | NM_001081491.2 | 56.6% | 55.5% | (many diffs) |
| 3 | human | 10482 | NXF1 | nuclear RNA export factor 1 | XR_001747724.2 | 44.2% | (many diffs) | |
| 4 | mouse | 53319 | Nxf1 | nuclear RNA export factor 1 | NM_016813.2 | 87.4% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1923
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgagggg aagtcgtaca gcgaacacga tgatgaacgc gttaatttcc 121 ctcaaagaaa gaagaaaggc cggggtccct tccggtggaa atatggtgaa ggaaaccgta 181 ggtctggaag aggcggttct ggtattcggt cttcccgcct tgaggaagat gatggagatg 241 tggcaatgag tgatgcccag gatggtcccc gagtacgata caacccctat accacccgac 301 ctaaccgtcg gggtgatact tggcatgatc gagatcgcat tcatgttact gtgcggagag 361 acagagctcc tccagagaga ggaggggctg gcaccagcca ggatgggacc tcaaagaact 421 ggttcaagat tacaattcct tatggcagaa agtatgacaa ggcatggctc ctgagcatga 481 ttcagagcaa gtgcagtgtg cccttcaccc ctattgagtt tcactatgag aatacacggg 541 cccagttctt cgttgaagac gccagtactg cctctgcatt gaaggctgtc aactataaga 601 ttttggatcg ggagaaccga aggatatcta tcatcatcaa ctcttctgct ccaccccaca 661 ctatactgaa tgaactgaag ccagaacaag tagaacagct aaagctgatc atgagcaaac 721 gatacgatgg ctcccaacaa gcccttgacc tcaaaggcct ccgttcagac ccagatttgg 781 tggcccagaa cattgacgtt gtcctgaatc gcagaagctg tatggcagct accctgagga 841 tcattgaaga gaacatccct gagctattgt ccttgaactt gagcaacaac aggctgtaca 901 ggctggatga catgtctagc attgttcaga aggcacccaa cctgaagatc ctaaaccttt 961 ctggaaatga attgaagtct gagcgggaat tggacaagat aaaggggctg aagctagaag 1021 agctctggct cgatggaaac tccctgtgtg acaccttccg agaccagtcc acctacatca 1081 gcgccattcg cgaacgattt cccaagttac tacgcctgga tggccatgag ctacccccac 1141 caattgcctt tgatgttgaa gcccccacga cgttaccgcc ctgcaaggga agctattttg 1201 gaacagaaaa cttgaagagt ctggtcttgc acttcctgca acagtactat gcaatttacg 1261 actctggaga ccgacaaggg ctcctggatg cctaccatga tggggcctgc tgttccctga 1321 gcattccttt cattcctcag aaccctgccc gaagcagctt agccgagtat ttcaaggata 1381 gcagaaatgt gaagaagctt aaagacccta ccttgcggtt ccggctgctg aagcacacgc 1441 gtctcaacgt tgttgccttc ctcaatgagt tgcccaaaac ccagcacgac gtcaattccT 1501 TCGTGGTAGA CATAAGCGCC CAGACAAGCA CATTGCTGTG TTTTTCTGTC AATGGAGTCT 1561 TCAAGGAAGT GGACGGAAAG TCCCGGGATT CTTTGCGAGC CTTCACCCGG ACATTCATTG 1621 CTGTTCCTGC TAGCAATTCA GGGCTATGTA TTGTAAATGA TGAGCTATTT GTGCGGAATG 1681 CCAGTTCTGA AGAGATCCAA AGAGCCTTCG CTATGCCTGC ACCCACGCCT TCCTCCAGCC 1741 CGGTGCCCAC CCTCTCTCCA GAGCAGCAGG AAATGTTGCA AGCATTCTCT ACCCAGTCTG 1801 GCATGAACCT CGAGTGGTCC CAGAAGTGCC TTCAGGACAA CAACTGGGAC TACACCAGAT 1861 CTGCCCAGGC CTTCACTCAT CTCAAGGCCA AGGGCGAGAT CCCAGAAGTG GCATTCATGA 1921 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGACC ACTGTAACTG ATTCTGACAT GCACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt