Construct: ORF TRCN0000469056
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002834.1_s317c1
- Derived from:
- ccsbBroadEn_03873
- DNA Barcode:
- CCCTCCCTGTGCAAACTTCTAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNG7 (59284)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469056
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59284 | CACNG7 | calcium voltage-gated chann... | NM_031896.4 | 100% | 100% | |
| 2 | human | 59284 | CACNG7 | calcium voltage-gated chann... | XM_017027093.1 | 100% | 100% | |
| 3 | human | 59284 | CACNG7 | calcium voltage-gated chann... | XM_005259124.4 | 63.9% | 47.3% | 423_424ins146;680_915del |
| 4 | mouse | 81904 | Cacng7 | calcium channel, voltage-de... | NM_133189.3 | 90.9% | 100% | (many diffs) |
| 5 | mouse | 81904 | Cacng7 | calcium channel, voltage-de... | XM_006540441.3 | 90.9% | 100% | (many diffs) |
| 6 | mouse | 81904 | Cacng7 | calcium channel, voltage-de... | XM_017312406.1 | 56.2% | 62.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 894
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtcactgc agcagccgcg ccctgaccct gctgagcagc gtgtttggtg 121 cgtgtggcct gctcctggta ggcatcgcgg tcagcactga ctactggctg tacatggaag 181 aaggcacagt gctaccgcag aaccagacca ccgaggtcaa gatggccctg cacgccggcc 241 tctggcgagt ctgcttcttt gcaggtcggg agaaaggtcg ctgtgtggcc tcagaatatt 301 ttcttgaacc ggagatcaat ttggtgacgg aaaacacgga gaatattctg aagacagtgc 361 gcacggccac ccccttcccc atggtcagcc tcttcctcgt gttcacggcc ttcgtcatca 421 gcaacatcgg ccacatccgc ccgcagagga ccattctggc ttttgtctct ggcatcttct 481 tcatactatc gggcctctcc ttggtggtgg gcttggttct ttacatctcc agcatcaacg 541 acgaggtcat gaacaggccc agcagctctg agcagtattT TCATTATCGC TACGGGTGGT 601 CTTTTGCCTT CGCCGCTTCC TCCTTCCTAC TCAAAGAGGG GGCCGGCGTG ATGTCCGTGT 661 ACCTGTTCAC CAAGCGCTAC GCGGAGGAGG AGATGTACCG TCCACACCCG GCCTTCTACC 721 GCCCGCGTCT CAGCGACTGC TCCGACTACT CGGGCCAGTT CCTGCAGCCC GAGGCGTGGC 781 GCCGCGGCCG GAGCCCCTCC GACATCTCCA GCGACGTGTC CATCCAAATG ACGCAGAACT 841 ACCCTCCCGC CATCAAGTAC CCGGACCACC TGCACATCTC CACCTCGCCC TGCTTGCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAC CCTCCCTGTG CAAACTTCTA ATGACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att