Construct: ORF TRCN0000469070

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017088.1_s317c1
Derived from:
ccsbBroadEn_11052
DNA Barcode:
CTGTTAGTATTCACGCGCCCCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP2R5C (5527)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469070
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5527 PPP2R5C protein phosphatase 2 regul... NM_178587.3 85.5% 85.5% 1153_1347del
2 human 5527 PPP2R5C protein phosphatase 2 regul... XM_017021423.2 80.1% 80.1% 1153_1437del
3 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001352912.1 79.1% 79.1% 1153_1455del
4 human 5527 PPP2R5C protein phosphatase 2 regul... NM_178586.3 79.1% 79.1% 1153_1455del
5 human 5527 PPP2R5C protein phosphatase 2 regul... NM_002719.4 73.2% 73.2% 1153_1572del
6 human 5527 PPP2R5C protein phosphatase 2 regul... XM_005267824.1 68.6% 66.1% (many diffs)
7 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001161726.2 68.5% 66% (many diffs)
8 human 5527 PPP2R5C protein phosphatase 2 regul... XM_005267826.1 68.4% 67.5% (many diffs)
9 human 5527 PPP2R5C protein phosphatase 2 regul... XM_011536918.1 68.3% 66.9% (many diffs)
10 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001352914.2 67.9% 65.4% (many diffs)
11 human 5527 PPP2R5C protein phosphatase 2 regul... XM_005267822.3 66.6% 64.1% (many diffs)
12 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001161725.1 65.9% 65.5% (many diffs)
13 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001352913.2 63.9% 61.6% (many diffs)
14 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001352916.2 63.7% 63.7% 0_1ins225;928_1230del
15 human 5527 PPP2R5C protein phosphatase 2 regul... XM_005267819.1 63.4% 61.1% (many diffs)
16 human 5527 PPP2R5C protein phosphatase 2 regul... NM_001352915.1 58.9% 58.9% 0_1ins225;928_1347del
17 human 5527 PPP2R5C protein phosphatase 2 regul... XM_005267827.1 58.9% 58.9% 0_1ins225;928_1347del
18 human 5527 PPP2R5C protein phosphatase 2 regul... XM_011536920.1 58.9% 58.9% 0_1ins225;928_1347del
19 human 5528 PPP2R5D protein phosphatase 2 regul... NM_001270476.2 44.4% 53.4% (many diffs)
20 mouse 26931 Ppp2r5c protein phosphatase 2, regu... NM_001081458.2 77.5% 84% (many diffs)
21 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_017315084.1 76.6% 83.1% (many diffs)
22 mouse 26931 Ppp2r5c protein phosphatase 2, regu... NM_001081457.2 72.2% 78.3% (many diffs)
23 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_017315082.1 70.6% 76.6% (many diffs)
24 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_017315081.1 67.6% 73.3% (many diffs)
25 mouse 26931 Ppp2r5c protein phosphatase 2, regu... NM_001135001.1 66.9% 71.1% (many diffs)
26 mouse 26931 Ppp2r5c protein phosphatase 2, regu... NM_012023.3 66.8% 72.5% (many diffs)
27 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515923.3 66.2% 70.4% (many diffs)
28 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515920.1 63.8% 69.2% (many diffs)
29 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515922.2 62.8% 66.7% (many diffs)
30 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515919.2 61.6% 65.4% (many diffs)
31 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_017315086.1 61.3% 66.5% (many diffs)
32 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_017315085.1 59.9% 65.1% (many diffs)
33 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515918.2 59.2% 62.9% (many diffs)
34 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515917.1 58.6% 62.3% (many diffs)
35 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515924.2 56.2% 59.7% (many diffs)
36 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_017315083.1 54.1% 58.8% (many diffs)
37 mouse 26931 Ppp2r5c protein phosphatase 2, regu... XM_006515921.1 47.5% 50.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1218
ORF length:
1152
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt gacatgtaat aaagcgggca gcaggatggt ggtggatgcg gccaactcca
121atgggccttt ccagcccgtg gtccttctcc atattcgaga tgttcctcct gctgatcaag
181agaagctttt tatccagaag ttacgtcagt gttgcgtcct ctttgacttt gtttctgatc
241cactaagtga cctaaagtgg aaggaagtaa aacgagctgc tttaagtgaa atggtagaat
301atatcaccca taatcggaat gtgatcacag agcctattta cccagaagta gtccatatgt
361ttgcagttaa catgtttcga acattaccac cttcctccaa tcctacggga gcggaatttg
421acccggagga agatgaacca acgttagaag cagcctggcc tcatctacag cttgtttatg
481aatttttctt aagattttta gagtctccag atttccaacc taatatagcg aagaaatata
541ttgatcagaa gtttgtattg cagcttttag agctctttga cagtgaagat cctcgggaga
601gagattttct taaaaccacc cttcacagaa tctatgggaa attcctaggc ttgagagctt
661acatcagaaa acagataaat aatatatttt ataggtttat ttatgaaaca gagcatcata
721atggcatagc agagttactG GAAATATTGG GAAGTATAAT TAATGGATTT GCCTTACCAC
781TAAAAGAAGA GCACAAGATT TTCTTATTGA AGGTGTTACT ACCTTTGCAC AAAGTGAAAT
841CTCTGAGTGT CTACCATCCC CAGCTGGCAT ACTGTGTAGT GCAGTTTTTA GAAAAGGACA
901GCACCCTCAC GGAACCAGTG GTGATGGCAC TTCTCAAATA CTGGCCAAAG ACTCACAGTC
961CAAAAGAAGT AATGTTCTTA AACGAATTAG AAGAGATTTT AGATGTCATT GAACCATCAG
1021AATTTGTGAA GATCATGGAA CCCCTCTTCC GGCAGTTGGC CAAATGTGTC TCCAGCCCAC
1081ACTTCCAGGT GGCAGAGCGA GCTCTCTATT ACTGGAATAA TGAATACATC ATGAGTTTAA
1141TCAGTGACAA CGCAGCGAAG ATTCTGCCCA TCATGTTTCC TTCCTTGTAC CGCAACTCAA
1201AGACCCATTG GAACAAGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1261TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1321GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTGTTAG TATTCACGCG
1381CCCCCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)