Construct: ORF TRCN0000469072

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002573.1_s317c1
Derived from:
ccsbBroadEn_15878
DNA Barcode:
GGCTTATCCCGCCATCTCTTTTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACSM5 (54988)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469072
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54988 ACSM5 acyl-CoA synthetase medium ... NM_001324373.2 99.8% 99.5% 424A>G
2 human 54988 ACSM5 acyl-CoA synthetase medium ... NM_001324371.2 35.8% 35.7% 424A>G;625_1737del
3 human 54988 ACSM5 acyl-CoA synthetase medium ... NM_017888.3 35.8% 35.7% 424A>G;625_1737del
4 human 54988 ACSM5 acyl-CoA synthetase medium ... NM_001324372.2 35.6% 35.5% 424A>G;625_1746del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
690
ORF length:
624
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag accatggctg agacacctag tcctccaggc actgaggaac tccagggcat
121tctgtgggtc tcatgggaag ccagcacctc tacctgttcc tcagaagatc gtggccacct
181gggaagccat cagcctggga aggcagctgg tgcctgagta cttcaacttc gcccatgatg
241tgctGGATGT GTGGAGTCGG CTGGAAGAGG CTGGACACCG CCCCCCAAAT CCTGCCTTCT
301GGTGGGTCAA TGGCACAGGA GCAGAGATCA AGTGGAGCTT TGAGGAGCTG GGGAAGCAGT
361CCAGGAAGGC AGCCAATGTG CTGGGGGGTG CATGCGGCCT GCAGCCTGGG GACAGAATGA
421TGCTGGTACT CCCACGGCTC CCGGAGTGGT GGCTGGTCAG TGTGGCTTGC ATGCGGACAG
481GGACTGTGGT GATTCCGGGT GTGACTCAGC TGACAGAGAA GGACCTCAAG TACCGGCTGC
541AGGCGTCCAG GGCCAAGTCC ATTATCACCA GTGACTCCCT AGCTCCAAGG GTGGATGCCA
601TCAGTGCCGA ATGCCCCTCC CTCCAGACCA AGCTGCTGGT GTCAGACAGC AGTCGGCCAG
661GCTGGTTGAA CTTCAGGGAA CTCCTCCGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA
721TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
781CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGCTT
841ATCCCGCCAT CTCTTTTCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
901tgaaagatt

Download FASTA (ORF) (Full)