Construct: ORF TRCN0000469072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002573.1_s317c1
- Derived from:
- ccsbBroadEn_15878
- DNA Barcode:
- GGCTTATCCCGCCATCTCTTTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSM5 (54988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_001324373.2 | 99.8% | 99.5% | 424A>G |
| 2 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_001324371.2 | 35.8% | 35.7% | 424A>G;625_1737del |
| 3 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_017888.3 | 35.8% | 35.7% | 424A>G;625_1737del |
| 4 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_001324372.2 | 35.6% | 35.5% | 424A>G;625_1746del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag accatggctg agacacctag tcctccaggc actgaggaac tccagggcat 121 tctgtgggtc tcatgggaag ccagcacctc tacctgttcc tcagaagatc gtggccacct 181 gggaagccat cagcctggga aggcagctgg tgcctgagta cttcaacttc gcccatgatg 241 tgctGGATGT GTGGAGTCGG CTGGAAGAGG CTGGACACCG CCCCCCAAAT CCTGCCTTCT 301 GGTGGGTCAA TGGCACAGGA GCAGAGATCA AGTGGAGCTT TGAGGAGCTG GGGAAGCAGT 361 CCAGGAAGGC AGCCAATGTG CTGGGGGGTG CATGCGGCCT GCAGCCTGGG GACAGAATGA 421 TGCTGGTACT CCCACGGCTC CCGGAGTGGT GGCTGGTCAG TGTGGCTTGC ATGCGGACAG 481 GGACTGTGGT GATTCCGGGT GTGACTCAGC TGACAGAGAA GGACCTCAAG TACCGGCTGC 541 AGGCGTCCAG GGCCAAGTCC ATTATCACCA GTGACTCCCT AGCTCCAAGG GTGGATGCCA 601 TCAGTGCCGA ATGCCCCTCC CTCCAGACCA AGCTGCTGGT GTCAGACAGC AGTCGGCCAG 661 GCTGGTTGAA CTTCAGGGAA CTCCTCCGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGCTT 841 ATCCCGCCAT CTCTTTTCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt