Construct: ORF TRCN0000469076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004061.1_s317c1
- Derived from:
- ccsbBroadEn_03539
- DNA Barcode:
- GCTCATCATTTATGCTCCGCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM33 (55161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469076
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55161 | TMEM33 | transmembrane protein 33 | NM_018126.3 | 100% | 100% | |
| 2 | human | 55161 | TMEM33 | transmembrane protein 33 | XM_005248116.4 | 100% | 100% | |
| 3 | human | 55161 | TMEM33 | transmembrane protein 33 | XM_005248117.2 | 100% | 100% | |
| 4 | mouse | 67878 | Tmem33 | transmembrane protein 33 | NM_001285452.1 | 90.8% | 97.5% | (many diffs) |
| 5 | mouse | 67878 | Tmem33 | transmembrane protein 33 | NM_028975.4 | 90.8% | 97.5% | (many diffs) |
| 6 | mouse | 67878 | Tmem33 | transmembrane protein 33 | NM_030108.2 | 90.5% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agatacgacc ccgaacggcc cccaaggggc gggcgctgtg caattcatga 121 tgaccaataa actggacacg gcaatgtggc tttctcgctt gttcacagtt tactgctctg 181 ctctgtttgt tctgcctctt cttgggttgc atgaagcagc aagcttttac caacgtgctt 241 tgctggcaaa tgctcttacc agtgctctga ggctgcatca aagattacca cacttccagt 301 taagcagagc attcctggcc caggctttgt tagaggacag ctgccactac ctgttgtatt 361 cactcatctt tgtaaattcc tatccagtta caatgagtat cttcccagtc ttgttattct 421 cttTGCTTCA TGCTGCCACA TATACGAAAA AGGTCCTTGA CGCAAGGGGC TCAAATAGTT 481 TACCTCTGCT GAGATCTGTC TTGGACAAAT TAAGTGCTAA TCAACAAAAT ATTCTGAAAT 541 TCATTGCTTG CAATGAAATA TTCCTGATGC CTGCGACAGT TTTTATGCTT TTTAGTGGTC 601 AAGGAAGTTT GCTCCAACCT TTTATATACT ATAGATTTCT TACCCTTCGA TATTCGTCTC 661 GAAGAAACCC ATATTGTCGG ACCTTATTTA ATGAACTGAG GATTGTTGTT GAACACATAA 721 TAATGAAACC TGCTTGCCCA CTGTTTGTGA GAAGACTTTG TCTCCAGAGC ATTGCCTTTA 781 TAAGCAGATT GGCACCAACA GTTCCATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCTCATCA 961 TTTATGCTCC GCAGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt