Construct: ORF TRCN0000469081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009695.1_s317c1
- Derived from:
- ccsbBroadEn_16115
- DNA Barcode:
- TTCTGATTTTAACTGGTTACGTTA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEDD2 (162989)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162989 | DEDD2 | death effector domain conta... | XM_011526572.1 | 72.1% | 72.2% | 433_447del;570_768del |
| 2 | human | 162989 | DEDD2 | death effector domain conta... | XR_935758.2 | 62.8% | 1_123del;556_570del;693_882del | |
| 3 | human | 162989 | DEDD2 | death effector domain conta... | XM_011526571.1 | 61.7% | 61.8% | 433_447del;570_897del |
| 4 | human | 162989 | DEDD2 | death effector domain conta... | NM_001270615.2 | 57.5% | 57.6% | 555_963del |
| 5 | human | 162989 | DEDD2 | death effector domain conta... | NM_001270614.2 | 56.6% | 56.7% | 433_447del;570_978del |
| 6 | human | 162989 | DEDD2 | death effector domain conta... | NM_133328.4 | 56.6% | 56.7% | 433_447del;570_978del |
| 7 | human | 162989 | DEDD2 | death effector domain conta... | XM_017026403.1 | 56.4% | 56.5% | 433_447del;570_981del |
| 8 | human | 162989 | DEDD2 | death effector domain conta... | XM_011526569.1 | 50.4% | 50.5% | 433_447del;570_1098del |
| 9 | human | 162989 | DEDD2 | death effector domain conta... | XM_017026402.1 | 50.4% | 50.5% | 433_447del;570_1098del |
| 10 | human | 162989 | DEDD2 | death effector domain conta... | XM_024451400.1 | 50.4% | 50.5% | 433_447del;570_1098del |
| 11 | human | 162989 | DEDD2 | death effector domain conta... | NR_073047.2 | 14.8% | (many diffs) | |
| 12 | human | 162989 | DEDD2 | death effector domain conta... | NR_073050.2 | 14.3% | (many diffs) | |
| 13 | human | 162989 | DEDD2 | death effector domain conta... | NR_073048.2 | 14.2% | (many diffs) | |
| 14 | human | 162989 | DEDD2 | death effector domain conta... | NR_073049.2 | 14% | (many diffs) | |
| 15 | human | 162989 | DEDD2 | death effector domain conta... | NR_073051.2 | 13.6% | (many diffs) | |
| 16 | human | 162989 | DEDD2 | death effector domain conta... | NR_073046.2 | 13.5% | (many diffs) | |
| 17 | mouse | 67379 | Dedd2 | death effector domain-conta... | NM_207677.3 | 48.5% | 50.3% | (many diffs) |
| 18 | mouse | 67379 | Dedd2 | death effector domain-conta... | XM_006540322.1 | 48.5% | 50.3% | (many diffs) |
| 19 | mouse | 67379 | Dedd2 | death effector domain-conta... | XR_391094.2 | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctatccggg tcgaccccgg ccccgtgctg ggaggaggat gagtgcctgg 121 actactacgg gatgctgtcg cttcaccgta tgttcgaggt ggtgggcggg caactgaccg 181 agtgcgagct ggagctcctg gcctttctgc tggatgaggc tcctGGCGCC GCCGGAGGCT 241 TAGCCCGGGC CCGCAGCGGC CTAGAGCTCC TGCTGGAGCT GGAGCGCCGC GGGCAGTGCG 301 ACGAGAGCAA CCTGCGGCTG CTGGGGCAAC TCCTGCGCGT GCTGGCCCGC CACGACCTGC 361 TGCCGCACCT GGCGCGCAAG CGGCGCCGGC CAGTGTCTCC AGAACGCTAT AGCTATGGCA 421 CCTCCAGCTC TTCAAAGAGG ACAGAGGGTA GCTGCCGTCG CCGTCGGCAG TCAAGCAGTT 481 CTGCAAATTC TCAGCAGGGC TCCCCCCCAA CCAAGCGGCA GCGGCGGAGT CGGGGCCGGC 541 CCAGTGGTGG TGCCAGACGG CGGCGGAGAG GGGCCCCAGC CGCACCCCAG CAGCAGTCAG 601 AGCCCGCCAG ACCTTCCTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 661 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 721 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTCTG ATTTTAACTG 781 GTTACGTTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt