Construct: ORF TRCN0000469094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000156.1_s317c1
- Derived from:
- ccsbBroadEn_09832
- DNA Barcode:
- TCAGACATTCCTATTACTAAGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC44A5 (204962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469094
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 204962 | SLC44A5 | solute carrier family 44 me... | NM_152697.5 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_017000609.1 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_017000610.1 | 99.8% | 99.8% | (many diffs) |
| 4 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_017000611.1 | 99.8% | 99.8% | (many diffs) |
| 5 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_017000607.1 | 98.6% | 98.3% | (many diffs) |
| 6 | human | 204962 | SLC44A5 | solute carrier family 44 me... | NM_001320283.2 | 98.1% | 98.1% | (many diffs) |
| 7 | human | 204962 | SLC44A5 | solute carrier family 44 me... | NM_001130058.2 | 97.1% | 97.2% | (many diffs) |
| 8 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_017000608.1 | 95.9% | 95.7% | (many diffs) |
| 9 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_006710445.3 | 93.9% | 94% | (many diffs) |
| 10 | human | 204962 | SLC44A5 | solute carrier family 44 me... | XM_011540987.2 | 93.9% | 94% | (many diffs) |
| 11 | human | 204962 | SLC44A5 | solute carrier family 44 me... | NM_001320285.2 | 91.3% | 91.3% | (many diffs) |
| 12 | human | 204962 | SLC44A5 | solute carrier family 44 me... | NM_001320287.2 | 91.3% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2223
- ORF length:
- 2157
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgacacagaa aaaccagcag atactccctc tgaggaagag gactttggtg 121 atccaaggac atatgaccca gatttcaagg ggcctgttgc caacaggagt tgtacagatg 181 ttctgtgctg tatgatcttc ctactgtgta ttattggcta cattgtttta ggacttgtgg 241 cctgggtaca tggggacccc agaagagcag cctatcctac agacagccag ggccactttt 301 gtggccagaa gggcactccc aatgagaaca agaccatttt gttttacttt aacctgttac 361 gctgtaccag tccctccgta ttgctaaacc tacagtgccc taccacacag atctgtgtct 421 ccaagtgccc agaaaaattt ttaacctatg tggaaatgca acttttgtac acaaaagaca 481 aaagctactg ggaagactac cgtcagttct gtaagaccac tgctaagcct gtgaagtctc 541 tcacacagct tttactggat gatgattgtc caacagcgat ttttcccagc aaaccttttc 601 tccagagatg tttccctgac ttctctacca aaaatggcac tttaacaata ggaagtaaga 661 tgatgtttca agatggaaat ggagggacaa gaagtgttgt agaactcggg attgctgcaa 721 atggtatcaa taaacttctt gatgcaaagt cacttggatt gaaagtgttt gaagactatg 781 caagaacttg gtattggatt ctcattggcc tgacgattgc catggtcctt agttggatat 841 ttttgatact tctgaggttc atagctggat gcctcttctg ggtcttcatg attggtgtga 901 ttggaattat aggttatgga atatggcact gttaccagca gtacaccaat cttcaggaac 961 gcccaagttc tgtattaact atctatgaca tcgggattca gactaacata agcatgtact 1021 ttgaactgca acaaacatgg ttcacattta tgataatact ctgcatcatt gaagtgattg 1081 tcatcctcat gctgatcttc ctcaggaatc gaatccgagt cgccattatc ctgctgaagg 1141 aaggaagcaa agccattgga tatgttccta gtacattagt ctatccagct ttaactttca 1201 ttttgctctc aatctgcatt tgctactggg tcgtgacagc agttttcttg gcgacatcgg 1261 gggtacctgt atacaaagtc atagctccag gggggcattg tatacatgaa aatcaaacct 1321 gtgacccaga gatttttaat acaactgaaa ttgccaaagc ttgccctggg gctctgtgta 1381 actttgcttt ctatggtgga aagagcttgt accatcagta catccctacc ttccatgtat 1441 acaacttatt tgtctttctc tggcttataa acttcgtcat tgcattaggt cagtgcgccc 1501 ttgctggtgc attcgctact tattactggg ccatgaaaaa acctgatgac atcccacgat 1561 atccactttt tactgcattt ggacgagcca tacgatatca cacaggatcc ctagcatttg 1621 gatctttaat tattgcatta attcaaatgt ttaaaattgt actagaatac ttggaccacc 1681 gtcttaaacg tacccagaac acattgtcta aattcctaca gtgctgcctg agatgctgct 1741 tctggtgttt ggaaaatgca ataaagtttt taaacagaaa tgcctatatt atgattgcaa 1801 tatatGGCAG AAACTTCTGC AGGTCAGCAA AAGATGCTTT CAATCTGCTG ATGAGAAATG 1861 TTTTGAAAGT TGCAGTTACA GATGAAGTTA CATACTTTGT ATTATTCCTG GGGAAACTTC 1921 TAGTTGCTGG AAGTATAGGT GTTCTGGCCT TCCTATTCTT CACACAAAGA CTGCCAGTGA 1981 TTGCACAAGG ACCAGCATCT TTATATTACT ACTGGGTACC TTTGCTGACA GTCATTTTTG 2041 GGTCTTACCT GATTGCACAT GGGTTCTTCA GCGTCTATGC AATGTGTGTT GAAACAATTT 2101 TCATCTGCTT CTGTGAAGAT CTGGAAAGAA ATGATGGATC CACAGAAAAA CCCTACTTCG 2161 TAACCCCTAA CCTGCATGGA ATTCTGATCA AGAAGCAACT AGTTCCCCAG AAGCAGAAAG 2221 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2281 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2341 GGCTTTATAT ATCTTGTGGA AAGGACGATC AGACATTCCT ATTACTAAGA TCACGCGTTA 2401 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt