Construct: ORF TRCN0000469095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006603.1_s317c1
- Derived from:
- ccsbBroadEn_12155
- DNA Barcode:
- CCACTGTATCTTGTTACTTGGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM51 (55092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469095
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55092 | TMEM51 | transmembrane protein 51 | NM_001319665.2 | 99.7% | 100% | 393A>T |
| 2 | human | 55092 | TMEM51 | transmembrane protein 51 | NM_001136216.2 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
| 3 | human | 55092 | TMEM51 | transmembrane protein 51 | NM_001136217.1 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
| 4 | human | 55092 | TMEM51 | transmembrane protein 51 | NM_001136218.2 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
| 5 | human | 55092 | TMEM51 | transmembrane protein 51 | NM_018022.3 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
| 6 | human | 55092 | TMEM51 | transmembrane protein 51 | XM_005245919.1 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
| 7 | human | 55092 | TMEM51 | transmembrane protein 51 | XM_011541676.1 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
| 8 | human | 55092 | TMEM51 | transmembrane protein 51 | XM_017001590.1 | 53.2% | 45% | 342_345delCAGC;397A>T;410_759del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 495
- ORF length:
- 405
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATGGCCCA GTCCAAGGCC AATGGCTCGC 121 ACTATGCGCT GACCGCCATC GGCCTGGGGA TGCTGGTCCT TGGGGTGATC ATGGCCATGT 181 GGAACCTGGT ACCCGGCTTC AGCGCGGCCG AGAAGCCAAC AGCTCAGGGC AGCAACAAGA 241 CCGAGGTGGG TGGCGGCATC CTCAAGAGCA AGACCTTCTC TGTGGCCTAC GTGCTGGTCG 301 GGGCCGGGGT GATGCTGCTG CTGCTTTCTA TCTGCCTGAG TATCAGGGAT AAGAGGAAGC 361 AGCGGCAGGG CGAGGACCTG GCCCATGTCC AGCACCCGAC AGGCGCTGGG CCTCACGCCC 421 AGGAGGAAGA CAGGAGGAAG AAGAGGAGGA TGAGGAGGCT GCCTCAAGGT ACTATGTTCC 481 CTGCTACGAG GAAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCACTGTATC TTGTTACTTG 661 GTCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt