Construct: ORF TRCN0000469139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002863.1_s317c1
- Derived from:
- ccsbBroadEn_15979
- DNA Barcode:
- ACCGGTCTCGCCTGCAGCTCAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FTO (79068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469139
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363905.1 | 15.9% | 15.8% | 1_839del;850_851insGGGCAGAGAGGAGTGTT |
| 2 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363900.1 | 11.7% | 11.5% | 1_1208del;1219_1220insGGGCAGAGAGGAGTGTT |
| 3 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363901.1 | 11.7% | 11.5% | 1_1208del;1219_1220insGGGCAGAGAGGAGTGTT |
| 4 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363898.1 | 11.4% | 11.3% | 1_1238del;1249_1250insGGGCAGAGAGGAGTGTT |
| 5 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363899.1 | 11.4% | 11.3% | 1_1238del;1249_1250insGGGCAGAGAGGAGTGTT |
| 6 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363896.1 | 11.4% | 10.6% | (many diffs) |
| 7 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363897.1 | 11.2% | 11% | 1_1274del;1285_1286insGGGCAGAGAGGAGTGTT |
| 8 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001080432.3 | 10.6% | 10.4% | 1_1352del;1363_1364insGGGCAGAGAGGAGTGTT |
| 9 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363891.1 | 10.4% | 10.2% | 1_1382del;1393_1394insGGGCAGAGAGGAGTGTT |
| 10 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363894.1 | 10.2% | 10% | 1_1415del;1426_1427insGGGCAGAGAGGAGTGTT |
| 11 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NR_156761.1 | 4.8% | 1_602del;613_614insGGGCAGAGAGGAGTGTT;766_3320del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 239
- ORF end:
- 419
- ORF length:
- 180
- Sequence:
-
1 TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG 61 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT 121 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 181 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 241 GGCATGCCAG GGCAGAGAGG AGTGTTGGTG CCAGTCACGA ATTGCCCGAA CATTACCTGC 301 TGATCAGAAG CCAGAATGTC GGCCATACTG GGAAAAGGAT GATGCTTCGA TGCCTCTGCC 361 GTTTGACCTC ACAGACATCG TTTCAGAACT CAGAGGTCAG CTTCTGGAAG CAAAACCCTA 421 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 481 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 541 TTATATATCT TGTGGAAAGG ACGAACCGGT CTCGCCTGCA GCTCAAGTAC GCGTTAAGTC 601 gacaatcaac ctctggatta caaaatttgt gaaagatt