Construct: ORF TRCN0000469139

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002863.1_s317c1
Derived from:
ccsbBroadEn_15979
DNA Barcode:
ACCGGTCTCGCCTGCAGCTCAAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FTO (79068)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469139
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363905.1 15.9% 15.8% 1_839del;850_851insGGGCAGAGAGGAGTGTT
2 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363900.1 11.7% 11.5% 1_1208del;1219_1220insGGGCAGAGAGGAGTGTT
3 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363901.1 11.7% 11.5% 1_1208del;1219_1220insGGGCAGAGAGGAGTGTT
4 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363898.1 11.4% 11.3% 1_1238del;1249_1250insGGGCAGAGAGGAGTGTT
5 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363899.1 11.4% 11.3% 1_1238del;1249_1250insGGGCAGAGAGGAGTGTT
6 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363896.1 11.4% 10.6% (many diffs)
7 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363897.1 11.2% 11% 1_1274del;1285_1286insGGGCAGAGAGGAGTGTT
8 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001080432.3 10.6% 10.4% 1_1352del;1363_1364insGGGCAGAGAGGAGTGTT
9 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363891.1 10.4% 10.2% 1_1382del;1393_1394insGGGCAGAGAGGAGTGTT
10 human 79068 FTO FTO alpha-ketoglutarate dep... NM_001363894.1 10.2% 10% 1_1415del;1426_1427insGGGCAGAGAGGAGTGTT
11 human 79068 FTO FTO alpha-ketoglutarate dep... NR_156761.1 4.8% 1_602del;613_614insGGGCAGAGAGGAGTGTT;766_3320del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
239
ORF end:
419
ORF length:
180
Sequence:
1TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG
61GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT
121GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA
181TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT
241GGCATGCCAG GGCAGAGAGG AGTGTTGGTG CCAGTCACGA ATTGCCCGAA CATTACCTGC
301TGATCAGAAG CCAGAATGTC GGCCATACTG GGAAAAGGAT GATGCTTCGA TGCCTCTGCC
361GTTTGACCTC ACAGACATCG TTTCAGAACT CAGAGGTCAG CTTCTGGAAG CAAAACCCTA
421CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG
481TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT
541TTATATATCT TGTGGAAAGG ACGAACCGGT CTCGCCTGCA GCTCAAGTAC GCGTTAAGTC
601gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)