Transcript: Human NM_001080432.3

Homo sapiens FTO alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FTO (79068)
Length:
11573
CDS:
30..1547

Additional Resources:

NCBI RefSeq record:
NM_001080432.3
NBCI Gene record:
FTO (79068)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001080432.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000246249 CGGTTCACAACCTCGGTTTAG pLKO_005 962 CDS 100% 10.800 15.120 N FTO n/a
2 TRCN0000255405 TCGCATGGCAGCAAGCTAAAT pLKO_005 3792 3UTR 100% 13.200 10.560 N FTO n/a
3 TRCN0000255403 ACCTGAACACCAGGCTCTTTA pLKO_005 352 CDS 100% 13.200 9.240 N FTO n/a
4 TRCN0000246250 TCACCAAGGAGACTGCTATTT pLKO_005 896 CDS 100% 13.200 9.240 N FTO n/a
5 TRCN0000246247 TCACGAATTGCCCGAACATTA pLKO_005 1401 CDS 100% 13.200 9.240 N FTO n/a
6 TRCN0000246248 CCCATTAGGTGCCCATATTTA pLKO_005 3368 3UTR 100% 15.000 9.000 N FTO n/a
7 TRCN0000255404 TCTCGCATCCTCATTGGTAAT pLKO_005 312 CDS 100% 10.800 6.480 N FTO n/a
8 TRCN0000257473 GCCAGTGAAAGGGTCTAATAT pLKO_005 383 CDS 100% 15.000 7.500 Y FTO n/a
9 TRCN0000255402 CAACGTAACTTTGCTGAATTT pLKO_005 626 CDS 100% 13.200 6.600 Y FTO n/a
10 TRCN0000165205 GCCTCAGTTTCCTCATCTGTA pLKO.1 2631 3UTR 100% 4.950 2.475 Y YIF1B n/a
11 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 3564 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001080432.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15979 pDONR223 0% 10.6% 10.4% None 1_1352del;1363_1364insGGGCAGAGAGGAGTGTT n/a
2 ccsbBroad304_15979 pLX_304 0% 10.6% 10.4% V5 1_1352del;1363_1364insGGGCAGAGAGGAGTGTT n/a
3 TRCN0000469139 ACCGGTCTCGCCTGCAGCTCAAGT pLX_317 100% 10.6% 10.4% V5 1_1352del;1363_1364insGGGCAGAGAGGAGTGTT n/a
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