Transcript: Human NR_156761.1

Homo sapiens FTO alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant 13, non-coding RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FTO (79068)
Length:
3320
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_156761.1
NBCI Gene record:
FTO (79068)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_156761.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255405 TCGCATGGCAGCAAGCTAAAT pLKO_005 3013 3UTR 100% 13.200 10.560 N FTO n/a
2 TRCN0000246250 TCACCAAGGAGACTGCTATTT pLKO_005 461 3UTR 100% 13.200 9.240 N FTO n/a
3 TRCN0000246247 TCACGAATTGCCCGAACATTA pLKO_005 622 3UTR 100% 13.200 9.240 N FTO n/a
4 TRCN0000246248 CCCATTAGGTGCCCATATTTA pLKO_005 2589 3UTR 100% 15.000 9.000 N FTO n/a
5 TRCN0000165205 GCCTCAGTTTCCTCATCTGTA pLKO.1 1852 3UTR 100% 4.950 2.475 Y YIF1B n/a
6 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 2785 3UTR 100% 2.640 1.320 Y LINC01098 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_156761.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15979 pDONR223 0% 4.8% None 1_602del;613_614insGGGCAGAGAGGAGTGTT;766_3320del n/a
2 ccsbBroad304_15979 pLX_304 0% 4.8% V5 1_602del;613_614insGGGCAGAGAGGAGTGTT;766_3320del n/a
3 TRCN0000469139 ACCGGTCTCGCCTGCAGCTCAAGT pLX_317 100% 4.8% V5 1_602del;613_614insGGGCAGAGAGGAGTGTT;766_3320del n/a
Download CSV