Construct: ORF TRCN0000469150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005622.1_s317c1
- Derived from:
- ccsbBroadEn_03637
- DNA Barcode:
- CTGTCAAGGCCTGTTGGTCGCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOK4 (55715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55715 | DOK4 | docking protein 4 | NM_001369619.1 | 100% | 100% | |
| 2 | human | 55715 | DOK4 | docking protein 4 | NM_001369621.1 | 100% | 100% | |
| 3 | human | 55715 | DOK4 | docking protein 4 | NM_018110.5 | 100% | 100% | |
| 4 | human | 55715 | DOK4 | docking protein 4 | NM_001330556.2 | 89.3% | 89.3% | 862_978del |
| 5 | human | 55715 | DOK4 | docking protein 4 | NM_001369618.1 | 89.3% | 89.3% | 862_978del |
| 6 | human | 55715 | DOK4 | docking protein 4 | NM_001369620.1 | 89.3% | 89.3% | 862_978del |
| 7 | human | 55715 | DOK4 | docking protein 4 | XM_024450347.1 | 89.3% | 89.3% | 862_978del |
| 8 | human | 55715 | DOK4 | docking protein 4 | XM_024450348.1 | 89.3% | 89.3% | 862_978del |
| 9 | mouse | 114255 | Dok4 | docking protein 4 | NM_053246.3 | 91% | 98.1% | (many diffs) |
| 10 | mouse | 114255 | Dok4 | docking protein 4 | XM_006530579.3 | 91% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccaatttc agtgacatcg tcaagcaagg ctacgtgaag atgaagagca 121 ggaagctcgg gatctaccgg aggtgctggc tggtgttccg gaaatcctcc agcaaggggc 181 cccagcggct ggagaagtat ccagatgaga agtcggtgtg cctccggggc tgccccaagg 241 tgactgagat cagcaacgtc aagtgtgtta cgcggctccc caaggagacc aagcggcagg 301 cggtggccat catattcact gatgactcgg cacgtacctt cacctgcgac tcagagctag 361 aggcagagga gtggtacaag acactatctg tggagtgtct ggggtcccgc ctcaacgaca 421 tcagtctggg agaacctgac ctcctggccc caggggtgca gtgtgaacag acagatcgct 481 tcaatgtctt cctgctgccc tgccccaacc tggacgtgta tggcgagtgc aagctgcaga 541 tcacccacga gaacatctac ctctgggaca tccacaaccc ccgtgtgaag ctcgtctcgt 601 ggcccctctg ctcactgcgc cgctatggcc gggatgccac acgctttacc ttcgaggctg 661 gccggatgtg tgatgctggg gaaggactct ataccTTCCA GACACAAGAG GGGGAGCAGA 721 TTTACCAGCG CGTCCACAGT GCCACCCTGG CCATCGCAGA GCAGCACAAG CGGGTCCTGC 781 TGGAAATGGA GAAGAACGTG AGGCTGCTGA ACAAGGGCAC GGAACATTAC TCGTATCCCT 841 GCACACCCAC GACCATGCTG CCGCGCAGTG CCTACTGGCA CCACATCACT GGTTCCCAGA 901 ACATCGCCGA AGCCTCCAGC TATGCTGGTG AGGGGTATGG GGCAGCCCAG GCCAGCTCGG 961 AAACAGACCT CCTCAACAGA TTCATCCTGC TAAAGCCAAA GCCCAGCCAG GGGGACAGCA 1021 GTGAGGCCAA GACCCCATCC CAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGTCAAGGCC 1201 TGTTGGTCGC TAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att