Construct: ORF TRCN0000469155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014241.1_s317c1
- Derived from:
- ccsbBroadEn_15557
- DNA Barcode:
- AATCCCCCGACCGCGGCCGTCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYCR1 (5831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001282280.1 | 53.6% | 53.6% | 1_444del |
| 2 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_006907.4 | 53.5% | 53.6% | 1_444del;903A>G |
| 3 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_005256381.2 | 53.5% | 53.6% | 1_444del;903A>G |
| 4 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_011523583.2 | 53.5% | 53.6% | 1_444del;903A>G |
| 5 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_011523584.3 | 53.5% | 53.6% | 1_444del;903A>G |
| 6 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_024450849.1 | 53.5% | 53.6% | 1_444del;903A>G |
| 7 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001282281.2 | 49.3% | 49.4% | 1_525del;984A>G |
| 8 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_153824.3 | 47.9% | 47.5% | (many diffs) |
| 9 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001282279.2 | 43.7% | 43.8% | 1_444del;540_541ins93;810A>G |
| 10 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001330523.1 | 21.1% | 21% | (many diffs) |
| 11 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_011523585.2 | 19.5% | 19.3% | (many diffs) |
| 12 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_011248856.2 | 48.7% | 50.1% | (many diffs) |
| 13 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | NM_001348222.1 | 44.1% | 45.4% | (many diffs) |
| 14 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | NM_144795.3 | 44.1% | 45.4% | (many diffs) |
| 15 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_006532735.2 | 44.1% | 45.4% | (many diffs) |
| 16 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_011248855.2 | 44.1% | 45.4% | (many diffs) |
| 17 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_011248854.2 | 40.3% | 41.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 579
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcagctgctg agcagcgtgg gcttctgcac ggaggtggaa gaggacctga 121 ttgatgccgt cacggggctc agtggcagcg gccccgccta cgcattcaca gccctggatg 181 ccctggctga tgggggtgtg aagatgggac ttccaaggcg cctggcagtc cgcctcgggg 241 cccaggcccT CCTGGGGGCT GCCAAGATGC TGCTGCACTC AGAACAGCAC CCAGGCCAGC 301 TCAAGGACAA CGTCAGCTCT CCTGGTGGGG CCACCATCCA TGCCTTGCAT GTGCTGGAGA 361 GTGGGGGCTT CCGCTCCCTG CTCATCAACG CTGTGGAGGC CTCCTGCATC CGCACACGGG 421 AGCTGCAGTC CATGGCTGAC CAGGAGCAGG TGTCACCAGC CGCCATCAAG AAGACCATCC 481 TGGACAAGGT GAAGCTGGAC TCCCCTGCAG GGACCGCTCT GTCGCCTTCT GGCCACACCA 541 AGCTGCTCCC CCGCAGCCTG GCCCCAGCGG GCAAGGATTG CCCAACTTTC TTGTACAAAG 601 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 661 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 721 ACGAAATCCC CCGACCGCGG CCGTCCAGAC GCGTTAAGTC gacaatcaac ctctggatta 781 caaaatttgt gaaagatt