Construct: ORF TRCN0000469166
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013905.1_s317c1
- Derived from:
- ccsbBroadEn_06658
- DNA Barcode:
- AAATAATGTCAGCCTCCGGTTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NT5E (4907)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469166
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4907 | NT5E | 5'-nucleotidase ecto | NM_002526.4 | 99.8% | 99.6% | 1126A>G;1136T>C |
| 2 | human | 4907 | NT5E | 5'-nucleotidase ecto | NM_001204813.1 | 91.1% | 90.9% | 1126A>G;1136T>C;1209_1210ins150 |
| 3 | mouse | 23959 | Nt5e | 5' nucleotidase, ecto | NM_011851.4 | 85.3% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1788
- ORF length:
- 1722
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tccccgagcc gcgcgggcgc ccgcgacgct actcctcgcc ctgggcgcgg 121 tgctgtggcc tgcggctggc gcctgggagc ttacgatttt gcacaccaac gacgtgcaca 181 gccggctgga gcagaccagc gaggactcca gcaagtgcgt caacgccagc cgctgcatgg 241 gtggcgtggc tcggctcttc accaaggttc agcagatccg ccgcgccgaa cccaacgtgc 301 tgctgctgga cgccggcgac cagtaccagg gcactatctg gttcaccgtg tacaagggcg 361 ccgaggtggc gcacttcatg aacgccctgc gctacgatgc catggcactg ggaaatcatg 421 aatttgataa tggtgtggaa ggactgatcg agccactcct caaagaggcc aaatttccaa 481 ttctgagtgc aaacattaaa gcaaaggggc cactagcatc tcaaatatca ggactttatt 541 tgccatataa agttcttcct gttggtgatg aagttgtggg aatcgttgga tacacttcca 601 aagaaacccc ttttctctca aatccaggga caaatttagt gtttgaagat gaaatcactg 661 cattacaacc tgaagtagat aagttaaaaa ctctaaatgt gaacaaaatt attgcactgg 721 gacattcggg ttttgaaatg gataaactca tcgctcagaa agtgaggggt gtggacgtcg 781 tggtgggagg acactccaac acatttcttt acacaggcaa tccaccttcc aaagaggtgc 841 ctgctgggaa gtacccattc atagtcactt ctgatgatgg gcggaaggtt cctgtagtcc 901 aggcctatgc ttttggcaaa tacctaggct atctgaagat cgagtttgat gaaagaggaa 961 acgtcatctc ttcccatgga aatcccattc ttctaaacag cagcattcct gaagatccaa 1021 gcataaaagc agacattaac aaatggagga taaaattgga taattattct acccaggaat 1081 tagggaaaac aattgtctat ctggatggct cctctcaatc atgccgcttt agagaatgca 1141 acatgggcaa cctgatttgt gatgcaatga ttaacaacaa cctgagacac gcggatgaaa 1201 cgttctggaa ccacgtatcc atgtgcattt taaatggagg tggtatccgg tcgcccattg 1261 atgaacgcaa caatggcaca attacctggg agaacctggc tgctgtattg ccctttggag 1321 gcacatttga cctagtccag ttaaaaggtt ccaccctgaa gaaggCCTTT GAGCATAGCG 1381 TGCACCGCTA CGGCCAGTCC ACTGGAGAGT TCCTGCAGGT GGGCGGAATC CATGTGGTGT 1441 ATGATCTTTC CCGAAAACCT GGAGACAGAG TAGTCAAATT AGATGTTCTT TGCACCAAGT 1501 GTCGAGTGCC CAGTTATGAC CCTCTCAAAA TGGACGAGGT ATATAAGGTG ATCCTCCCAA 1561 ACTTCCTGGC CAATGGTGGA GATGGGTTCC AGATGATAAA AGATGAATTA TTAAGACATG 1621 ACTCTGGTGA CCAAGATATC AACGTGGTTT CTACATATAT CTCCAAAATG AAAGTAATTT 1681 ATCCAGCAGT TGAAGGTCGG ATCAAGTTTT CCACAGGAAG TCACTGCCAT GGAAGCTTTT 1741 CTTTAATATT TCTTTCACTT TGGGCAGTGA TCTTTGTTTT ATACCAATAC CCAACTTTCT 1801 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1861 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1921 GTGGAAAGGA CGAAAATAAT GTCAGCCTCC GGTTTAAACG CGTTAAGTCg acaatcaacc 1981 tctggattac aaaatttgtg aaagatt