Construct: ORF TRCN0000469167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017783.1_s317c1
- Derived from:
- ccsbBroadEn_06851
- DNA Barcode:
- TAAAAAGGTTGAGCGTTAAGCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBP3 (5949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5949 | RBP3 | retinol binding protein 3 | NM_002900.3 | 99.9% | 99.8% | 8G>N;2270T>G |
| 2 | mouse | 19661 | Rbp3 | retinol binding protein 3, ... | NM_015745.2 | 81.6% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3807
- ORF length:
- 3741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ganagaatgg gttctgctca tgtccgtgct gctctgtggc ctggctggcc 121 ccacacacct gttccagcca agcctggtgc tggacatggc caaggtcctc ttggataact 181 actgcttccc ggagaacctg ctgggcatgc aggaagccat ccagcaggcc atcaagagcc 241 atgagattct gagcatctca gacccgcaga cgctggccag tgtgctgaca gccggggtgc 301 agagctccct gaacgatcct cgcctggtca tctcctatga gcccagcacc cccgagcctc 361 ccccacaagt cccagcactc accagcctct cagaagagga actgcttgcc tggctgcaaa 421 ggggcctccg ccatgaggtt ctggagggta atgtgggcta cctgcgggtg gacagcgtcc 481 cgggccagga ggtgctgagc atgatggggg agttcctggt ggcccacgtg tgggggaatc 541 tcatgggcac ctccgcctta gtgctggatc tccggcactg cacaggaggc caggtctctg 601 gcattcccta catcatctcc tacctgcacc cagggaacac catcctgcac gtggacacta 661 tctacaaccg cccctccaac accaccacgg agatctggac cttgccccag gtcctgggag 721 aaaggtacgg tgccgacaag gatgtggtgg tcctcaccag cagccagacc aggggcgtgg 781 ccgaggacat cgcgcacatc cttaagcaga tgcgcagggc catcgtggtg ggcgagcgga 841 ctgggggagg ggccctggac ctccggaagc tgaggatagg cgagtctgac ttcttcttca 901 cggtgcccgt gtccaggtcc ctggggcccc ttggtggagg cagccagacg tgggagggca 961 gcggggtgct gccctgtgtg gggactccgg ccgagcaggc cctggagaaa gccctggcca 1021 tcctcactct gcgcagcgcc cttccagggg tagtccactg cctccaggag gtcctgaagg 1081 actactacac gctggtggac cgtgtgccca ccctgctgca gcacttggcc agcatggact 1141 tctccacggt ggtctccgag gaagatctgg tcaccaagct caatgccggc ctgcaggctg 1201 cgtctgagga tcccaggctc ctggtgcgag ccatcgggcc cacagaaact ccttcttggc 1261 ccgcgcccga cgctgcagcc gaagactcac caggggtggc cccagagttg cctgaggacg 1321 aggctatccg gcaagcactg gtggactctg tgttccaggt gtcggtgctg ccaggcaatg 1381 tgggctacct gcgcttcgat agttttgctg acgcctccgt cctgggtgtg ttggccccat 1441 atgtcctgcg ccaggtgtgg gagccgctac aggacacgga gcacctcatc atggacctgc 1501 gccacaaccc tggagggcca tcctctgctg tgcccctgct cctgtcctac ttccagggcc 1561 ctgaggccgg ccccgtgcac ctcttcacca cctatgatcg ccgcaccaac atcacgcagg 1621 agcacttcag ccacatggag ctcccgggcc cacgctacag cacccaacgt ggggtgtatc 1681 tgctcaccag ccaccgcacc gccacggccg cggaggagtt cgccttcctt atgcagtcgc 1741 tgggctgggc cacactggta ggtgagatca ccgcgggcaa cctgctgcac acccgcacgg 1801 tgccgctgct ggacacaccc gaaggcagcc tcgcgctcac cgtgccggtc ctcaccttca 1861 tcgacaatca cggcgaggcc tggctgggtg gtggagtggt gcccgatgcc atcgtgctgg 1921 ccgaggaggc cctggacaaa gcccaggaag tgctggagtt ccaccaaagc ctgggggcct 1981 tggtggaggg cacagggcac ctgctggagg cccactatgc tcggccagag gtcgtggggc 2041 agaccagtgc cctcctgcgg gccaagctgg cccagggcgc ctaccgcaca gctgtggact 2101 tggagtctct ggcctctcag ctcacagcag acctccagga ggtgtctggg gaccaccgct 2161 tgctagtgtt ccacagccct ggcgagctgg tggtagagga agcaccccca ccaccccctg 2221 ctgtcccctc tccagaggag ctcacctacc ttattgaggc cctgttcaag acagaggtgc 2281 tgcccggcca gctgggctac ctgcgttttg acgccatggc tgaactggag acagggaagg 2341 ccgtggggcc acagctggtg cggctggtat ggcaacagct ggtggacacg gctgcgctgg 2401 tgatcgacct gcgctacaac cctggcagct actccacggc catcccgctg ctctgctcct 2461 acttctttga ggcagagccc cgccagcacc tgtattctgt ctttgacagg gccacctcaa 2521 aagtcacgga ggtgtggacc ttgccccagg tcgccggcca gcgctacggc tcacacaagg 2581 acctctacat cctgatgagc cacaccagtg gctctgcggc cgaggccttt gcacacacca 2641 tgcaggacct gcagcgggcc acggtcattg gggagcccac ggccggaggc gcactctctg 2701 tgggcatcta ccaggtgggc agcagcccct tatatgcatc catgcccacc cagatggcca 2761 tgagtgccac cacaggcaag gcctgggacc tggctggtgt ggagcccgac atcactgtgc 2821 ccatgagcga agccctttcc atagcccagg acatagtggc tctgcgtgcc aaggtgccca 2881 cggtgctgca gacggccggg aagctggtgg ctgataacta tgcctctgcc gagctggggg 2941 ccaagatggc caccaaactg agcggtctgc agagccgcta ctccagggtg acctcagaag 3001 tggccctagc cgagatcctg ggggctgacc tgcagatgct ctccggagac ccacacctga 3061 aggcagccca tatccctgag aatgccaagg accgcattcc tggaattgtg cccatgcaga 3121 tcccttcccc tgaagtattt gaagagctga tcaagttttc cttccacact aacgtgcttg 3181 aggacaacat tggctacttg aggtttgaca tgtttgggga cggtgagctg ctcacccagg 3241 tctccaggct gctggtggag cacatctgga agaagatcat gcacacggat gccatgatca 3301 tcgacatgag gttcaacatc ggtggcccca catcctccat tcccatcttg tgctcctact 3361 tctttgatga aggccctcca gttctgctgg acaagatcta cagccggcct gatgactctg 3421 tcagtgaact ctggacacac gcccaggttg taggtgaacg ctatggctcc aagaagagca 3481 tggtcatTCT GACCAGCAGT GTGACGGCCG GCACCGCGGA GGAGTTCACC TATATCATGA 3541 AGAGGCTGGG CCGGGCCCTG GTCATTGGGG AGGTGACCAG TGGGGGCTGC CAGCCACCAC 3601 AGACCTACCA CGTGGATGAC ACCAACCTCT ACCTCACTAT CCCCACGGCC CGTTCTGTGG 3661 GGGCCTCGGA TGGCAGCTCC TGGGAAGGGG TGGGGGTGAC ACCCCATGTG GTTGTCCCTG 3721 CAGAAGAGGC TCTCGCCAGG GCCAAGGAGA TGCTCCAGCA CAACCAGCTG AGGGTGAAGC 3781 GGAGCCCAGG CCTGCAGGAC CACCTGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 3841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAAAAAGG 3961 TTGAGCGTTA AGCGAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4021 aagatt