Construct: ORF TRCN0000469171
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011644.1_s317c1
- Derived from:
- ccsbBroadEn_10402
- DNA Barcode:
- AAATCACCTGGGAAAGCCTAACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469171
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23521 | RPL13A | ribosomal protein L13a | NM_001270491.1 | 67.8% | 64% | (many diffs) |
| 2 | human | 23521 | RPL13A | ribosomal protein L13a | NM_012423.4 | 47.4% | 44.8% | (many diffs) |
| 3 | human | 728658 | RPL13AP5 | ribosomal protein L13a pseu... | NR_026712.1 | 44% | (many diffs) | |
| 4 | human | 387841 | RPL13AP20 | ribosomal protein L13a pseu... | NR_003932.2 | 43.3% | (many diffs) | |
| 5 | human | 644511 | RPL13AP6 | ribosomal protein L13a pseu... | NR_026715.1 | 42.5% | (many diffs) | |
| 6 | human | 23521 | RPL13A | ribosomal protein L13a | NR_073024.1 | 24.3% | (many diffs) | |
| 7 | human | 645683 | RPL13AP3 | ribosomal protein L13a pseu... | NR_004844.1 | 20.5% | 1_194del;501_1488del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 372
- ORF length:
- 306
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatGCT GCGCCACAAG ACCAAGCGAG GCCATGCCTC CCTGGACTGC CTCAAGGTGT 121 TTGACGGCAT CCCACCGCCC TACGATAAGA AAAAGCGGAT GGTGGTTCCT GCTGCCCTCA 181 AGGTCGTGCG TCTGAAGCCT ACAAGAAAGT TTGCCCTTCT GGGGCGACAG GCTCAAGAGG 241 TTAGGTGGAA GTACCAGGCA GTGACAGCCA CCCTGGAGGA GAAGAGGAAG GAGAAAGCCA 301 AGATCCACTA CTGGAAGAAG AAACAGCTCA TGAGGCTACG GAAACAGGCC GAGAAGAACG 361 TGAAGAAAAA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 421 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 481 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA TCACCTGGGA AAGCCTAACT 541 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t