Transcript: Human NM_001270491.1

Homo sapiens ribosomal protein L13a (RPL13A), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
RPL13A (23521)
Length:
1120
CDS:
184..612

Additional Resources:

NCBI RefSeq record:
NM_001270491.1
NBCI Gene record:
RPL13A (23521)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001270491.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415662 ACCAGTTACTATGAGTGAAAG pLKO_005 838 3UTR 100% 10.800 7.560 N RPL13A n/a
2 TRCN0000413904 GGAGCAAGGAAAGGGTCTTAG pLKO_005 739 3UTR 100% 10.800 7.560 N RPL13A n/a
3 TRCN0000430638 CTGATTGGAGGGCCCTATCTT pLKO_005 871 3UTR 100% 5.625 3.938 N RPL13A n/a
4 TRCN0000427978 GTGCAGGTGTCATTTATCTAT pLKO_005 800 3UTR 100% 5.625 3.938 N RPL13A n/a
5 TRCN0000117717 GCCCAATAAAGACTGTTAATT pLKO.1 613 CDS 100% 15.000 7.500 Y RPL13A n/a
6 TRCN0000117720 CCGGAAGAAGAAACAGCTCAT pLKO.1 504 CDS 100% 4.050 2.025 Y RPL13A n/a
7 TRCN0000117719 CCTACAAGAAAGTTTGCCTAT pLKO.1 391 CDS 100% 4.050 2.025 Y RPL13A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001270491.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02785 pDONR223 100% 69.9% 69.9% None 0_1ins183 n/a
2 ccsbBroad304_02785 pLX_304 0% 69.9% 69.9% V5 0_1ins183 n/a
3 TRCN0000475232 TTTGACACCCTGAAGGCCGTTTAT pLX_317 72.9% 69.9% 69.9% V5 0_1ins183 n/a
4 ccsbBroadEn_07895 pDONR223 100% 69.7% 69.9% None 0_1ins183;225C>T n/a
5 ccsbBroad304_07895 pLX_304 0% 69.7% 69.9% V5 0_1ins183;225C>T n/a
6 TRCN0000471523 CTACGTGCCCCGATATGGCGCTGG pLX_317 45.6% 69.7% 69.9% V5 0_1ins183;225C>T n/a
7 ccsbBroadEn_15764 pDONR223 0% 69.7% 69.9% None 0_1ins183;204G>T n/a
8 ccsbBroad304_15764 pLX_304 0% 69.7% 69.9% V5 0_1ins183;204G>T n/a
9 TRCN0000491289 CACAACGAAAACCTGGGTGGACTC pLX_317 45.6% 69.7% 69.9% V5 0_1ins183;204G>T n/a
10 ccsbBroadEn_10402 pDONR223 100% 67.8% 64% None (many diffs) n/a
11 ccsbBroad304_10402 pLX_304 0% 67.8% 64% V5 (many diffs) n/a
12 TRCN0000469171 AAATCACCTGGGAAAGCCTAACTT pLX_317 96.6% 67.8% 64% V5 (many diffs) n/a
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