Construct: ORF TRCN0000469203
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001764.1_s317c1
- Derived from:
- ccsbBroadEn_01130
- DNA Barcode:
- CATCAGGACGTAAAATTGATAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OVOL1 (5017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469203
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5017 | OVOL1 | ovo like transcriptional re... | NM_004561.4 | 100% | 100% | |
| 2 | human | 5017 | OVOL1 | ovo like transcriptional re... | XM_005274018.3 | 76.7% | 76.7% | 0_1ins186 |
| 3 | human | 5017 | OVOL1 | ovo like transcriptional re... | XM_011545067.2 | 76.7% | 76.7% | 0_1ins186 |
| 4 | human | 5017 | OVOL1 | ovo like transcriptional re... | XM_011545068.3 | 76.7% | 76.7% | 0_1ins186 |
| 5 | human | 5017 | OVOL1 | ovo like transcriptional re... | XM_017017837.1 | 76.7% | 76.7% | 0_1ins186 |
| 6 | human | 5017 | OVOL1 | ovo like transcriptional re... | XM_017017838.2 | 76.7% | 76.7% | 0_1ins186 |
| 7 | mouse | 18426 | Ovol1 | ovo like zinc finger 1 | NM_019935.3 | 90.1% | 95.5% | (many diffs) |
| 8 | mouse | 18426 | Ovol1 | ovo like zinc finger 1 | XM_011248605.2 | 78.6% | 83.1% | (many diffs) |
| 9 | mouse | 18426 | Ovol1 | ovo like zinc finger 1 | XM_006531675.3 | 68.7% | 73% | (many diffs) |
| 10 | mouse | 18426 | Ovol1 | ovo like zinc finger 1 | XM_006531676.3 | 68.7% | 73% | (many diffs) |
| 11 | mouse | 18426 | Ovol1 | ovo like zinc finger 1 | XM_011248606.2 | 68.7% | 73% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 867
- ORF length:
- 801
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccgcgcgttc ctggtgaaga agccgtgcgt ctccacgtgc aagaggaact 121 ggagcgagct ccccgacgag gagcgcggcg agatctacgt gccagtcagc ctgggcttct 181 gcccaccaca gccctaccgg gagccggaac cctctgtggc cgaaccccct tcctgcccgc 241 tggctttgaa catgagcctt cgagactcta gctacagcat ggcccccggg ccctgtgtgg 301 tggcccagct gccctctgaa gacatgggcc acttgacaga cccccagagc agagaccatg 361 gcttcctgcg caccaagatg aaggtgaccc ttggggacag tcccAGTGGA GACCTGTTCA 421 CCTGCCGTGT CTGCCAGAAG GCCTTCACCT ACCAGCGCAT GCTGAACCGC CACATGAAGT 481 GTCACAACGA CGTCAAGAGG CACCTCTGCA CGTACTGCGG GAAGGGCTTC AATGACACCT 541 TCGACCTCAA GAGACACGTC CGAACTCACA CTGGCGTGCG GCCCTACAAG TGCAGCCTGT 601 GTGACAAGGC CTTCACGCAG CGCTGCTCTC TGGAGTCTCA CCTCAAGAAG ATCCATGGTG 661 TGCAGCAGAA GTACGCGTAC AAGGAGCGGC GGGCCAAGCT GTACGTGTGT GAGGAGTGCG 721 GCTGCACATC TGAGAGCCAG GAGGGCCACG TCCTGCACCT GAAGGAGCAC CACCCTGACA 781 GCCCGCTGCT GCGCAAGACC TCCAAGAAGG TGGCCGTGGC ACTACAGAAC ACTGTCACTT 841 CCCTGCTGCA GGGCAGCCCC CACCTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 901 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 961 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACATCAGGA 1021 CGTAAAATTG ATAAGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1081 aagatt