Construct: ORF TRCN0000469206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010103.1_s317c1
- Derived from:
- ccsbBroadEn_12728
- DNA Barcode:
- TTTGATATTGTAGGTATGTGATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NETO2 (81831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469206
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81831 | NETO2 | neuropilin and tolloid like 2 | XM_017023740.1 | 38% | 38% | 1_723del |
| 2 | human | 81831 | NETO2 | neuropilin and tolloid like 2 | XM_006721291.1 | 30.8% | 30.8% | 1_996del |
| 3 | human | 81831 | NETO2 | neuropilin and tolloid like 2 | XM_006721289.1 | 30.3% | 30.3% | 1_1017del |
| 4 | human | 81831 | NETO2 | neuropilin and tolloid like 2 | NM_001201477.2 | 28.5% | 28.5% | 1_1110del |
| 5 | human | 81831 | NETO2 | neuropilin and tolloid like 2 | NM_018092.5 | 28.1% | 28.1% | 1_1131del |
| 6 | mouse | 74513 | Neto2 | neuropilin (NRP) and tolloi... | XM_006531440.2 | 25.9% | 27.7% | (many diffs) |
| 7 | mouse | 74513 | Neto2 | neuropilin (NRP) and tolloi... | XM_006531439.2 | 25.5% | 27.4% | (many diffs) |
| 8 | mouse | 74513 | Neto2 | neuropilin (NRP) and tolloi... | NM_001081324.1 | 24.2% | 26% | (many diffs) |
| 9 | mouse | 74513 | Neto2 | neuropilin (NRP) and tolloi... | XM_006531437.3 | 24.2% | 26% | (many diffs) |
| 10 | mouse | 74513 | Neto2 | neuropilin (NRP) and tolloi... | XM_011248528.1 | 23.9% | 25.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGC TTGCAAAACC GCTTTTAATA AAACCGGGTT CCAAGAAGTG TTTGATCCTC 121 CTCATTATGA ACTGTTTTCA CTAAGGGACA AAGAGATTTC TGCAGACCTG GCAGACTTGT 181 CGGAAGAATT GGACAACTAC CAGAAGATGC GGCGCTCCTC CACCGCCTCC CGCTGCATCC 241 ACGACCACCA CTGTGGGTCG CAGGCCTCCA GCGTCAAACA AAGCAGGACC AACCTCAGTT 301 CCATGGAACT TCCTTTCCGA AATGACTTTG CACAACCACA GCCAATGAAA ACATTTAATA 361 GCACCTTCAA GAAAAGTAGT TACACTTTCA AACAGGGACA TGAGTGCCCT GAGCAGGCCC 421 TGGAAGACCG AGTAATGGAG GAGATTCCCT GTGAAATTTA TGTCAGGGGG CGAGAAGATT 481 CTGCACAAGC ATCCATATCC ATTGACTTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTGA 661 TATTGTAGGT ATGTGATCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 721 tgaaagatt