Construct: ORF TRCN0000469208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016991.1_s317c1
- Derived from:
- ccsbBroadEn_16027
- DNA Barcode:
- ACTGCCTTGCTGATTTAACTCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACAD11 (84129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NM_032169.5 | 50% | 50% | 1_1167del;2329A>C |
| 2 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NR_132428.1 | 32.6% | (many diffs) | |
| 3 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NR_132426.1 | 28% | (many diffs) | |
| 4 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NR_132427.1 | 24.3% | (many diffs) | |
| 5 | human | 100532724 | NPHP3-ACAD11 | NPHP3-ACAD11 readthrough (N... | NR_037804.1 | 15% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acaacacatt cttccagctg aaaaggaggt aactgagttc tatgttcaaa 121 atgaaaattc agtggacaag tggggaaaac ctttagtgat tgataaactc aaggaaatgg 181 ccaaagtcga gggtctctgg aacttgtttt tgccagctgt cagcggactc agccacgtgg 241 actatgcctt gattgctgaa gaaacaggaa aatgcttttt tgctccagat gtctttaact 301 gccaagcacc agacacaggg aatatggagg ttctgcacct gtatggaagt gaggaacaga 361 agaaacagtg gcttgagcct cttcttcaag ggaacattac ctcttgcttc tgtatgacag 421 aacctgatgt agcttcaagt gatgccacga atattgaatg cagcatccaa cgagatgaag 481 atagctatgt aattaacggc aaaaaatggt ggagcagtgg agctgggaat cccaagtgca 541 aaattgcaat tgttttggga agaactcaaa atacttctct ctccagacac aaacagcaca 601 gcatgattct tgttcccatg aacacacctg gagtaaaaat aataaggcct ttgtcagttt 661 ttggctacac agataatttt catggaggac attttgagat ccattttaat caagtgcgag 721 ttcctgccac aaatctaata ctaggtgaag gtaggggatt tgaaatttcc caaggccgcc 781 ttggacctgg cagaatccac cactgtatga gaacagtagg tttggcggaa cgcgctttgc 841 agatcatgtg tgagcgggca acacaaagga tagctttcaa gaagaagttG TATGCACATG 901 AGGTTGTGGC TCACTGGATT GCTGAAAGCC GCATTGCCAT TGAGAAGATC CGCTTGTTGA 961 CTCTGAAAGC TGCTCACAGC ATGGACACTC TGGGCAGTGC TGGCGCTAAG AAAGAGATTG 1021 CAATGATCAA AGTGGCTGCC CCACGGGCTG TCAGCAAAAT CGTTGACTGG GCCATCCAGG 1081 TGTGCGGAGG TGCTGGTGTT TCCCAGGATT ACCCTCTGGC TAACATGTAT GCTATAACCC 1141 GAGTTTTGCG TTTAGCAGAT GGACCTGACG AAGTTCATCT TTCAGCAATC GCAACAATGG 1201 AGCTGCGGGA CCAAGCCAAA AGACTGCCAG CCAAGATATA CCCAACTTTC TTGTACAAAG 1261 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1321 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1381 ACGAACTGCC TTGCTGATTT AACTCACAAC GCGTTAAGTC gacaatcaac ctctggatta 1441 caaaatttgt gaaagatt