Transcript: Human NR_037804.1

Homo sapiens NPHP3-ACAD11 readthrough (NMD candidate) (NPHP3-ACAD11), long non-coding RNA.

Source:
NCBI, updated 2018-09-16
Taxon:
Homo sapiens (human)
Gene:
NPHP3-ACAD11 (100532724)
Length:
7788
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_037804.1
NBCI Gene record:
NPHP3-ACAD11 (100532724)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_037804.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000366390 GTGCGAGACAATGGGATATTT pLKO_005 1088 3UTR 100% 15.000 7.500 Y Nphp3 n/a
2 TRCN0000423775 GTTTGAGCACAGGGTTAATTA pLKO_005 7047 3UTR 100% 15.000 7.500 Y ACAD11 n/a
3 TRCN0000118614 CCTCCCTTATTCACAGTTTAT pLKO.1 2405 3UTR 100% 13.200 6.600 Y NPHP3 n/a
4 TRCN0000028403 GCAGATATCTTCTGGGAAATA pLKO.1 4815 3UTR 100% 13.200 6.600 Y ACAD11 n/a
5 TRCN0000417096 GGTCGAATCTTCCGTGATTTA pLKO_005 4217 3UTR 100% 13.200 6.600 Y ACAD11 n/a
6 TRCN0000028439 GCATCCAACGAGATGAAGATA pLKO.1 5567 3UTR 100% 5.625 2.813 Y ACAD11 n/a
7 TRCN0000028431 GCTCAGTTACATTCCTTGAAT pLKO.1 4301 3UTR 100% 5.625 2.813 Y ACAD11 n/a
8 TRCN0000118615 GCTTAGCTGATCTTTATGAAA pLKO.1 2828 3UTR 100% 5.625 2.813 Y NPHP3 n/a
9 TRCN0000028422 GCACATCAGATTGATAGAGAA pLKO.1 4082 3UTR 100% 4.950 2.475 Y ACAD11 n/a
10 TRCN0000028453 GCTGGCGCTAAGAAAGAGATT pLKO.1 6713 3UTR 100% 4.950 2.475 Y ACAD11 n/a
11 TRCN0000118613 GCTGTTGAAATTCGACAGAAA pLKO.1 3613 3UTR 100% 4.950 2.475 Y NPHP3 n/a
12 TRCN0000118616 GCCTTGGTGAACTTAGCTGTT pLKO.1 3667 3UTR 100% 4.050 2.025 Y NPHP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_037804.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04332 pDONR223 100% 29.2% None (many diffs) n/a
2 ccsbBroad304_04332 pLX_304 0% 29.2% V5 (many diffs) n/a
3 TRCN0000475369 CGGATCTAAAGCTCTTGGTTCGTA pLX_317 19.5% 29.2% V5 (many diffs) n/a
4 ccsbBroadEn_16027 pDONR223 0% 15% None (many diffs) n/a
5 ccsbBroad304_16027 pLX_304 0% 15% V5 (many diffs) n/a
6 TRCN0000469208 ACTGCCTTGCTGATTTAACTCACA pLX_317 30.1% 15% V5 (many diffs) n/a
7 ccsbBroadEn_11839 pDONR223 100% 5.5% None (many diffs) n/a
8 ccsbBroad304_11839 pLX_304 0% 5.5% V5 (many diffs) n/a
9 TRCN0000470761 CTATACACCATTGACTGTCGGCCA pLX_317 72.9% 5.5% V5 (many diffs) n/a
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