Construct: ORF TRCN0000469215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017100.1_s317c1
- Derived from:
- ccsbBroadEn_14002
- DNA Barcode:
- TTAGGTTACCGGTGGACTGATAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX3 (8724)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469215
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8724 | SNX3 | sorting nexin 3 | NM_001300928.2 | 83.1% | 77.1% | (many diffs) |
| 2 | human | 8724 | SNX3 | sorting nexin 3 | NM_003795.6 | 64.5% | 54.1% | (many diffs) |
| 3 | human | 8724 | SNX3 | sorting nexin 3 | NM_001300929.2 | 51.1% | 41.1% | (many diffs) |
| 4 | mouse | 54198 | Snx3 | sorting nexin 3 | NM_017472.4 | 61.8% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 429
- ORF length:
- 339
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGACA TGGCGGAGAC CGTGGCTGAC ACCCGGCGGC 121 TGATCACCAA GCCGCAGAAC CTGAATGACG CCTACGGACC CCCCAGCAAC TTCCTCGAGA 181 TCGATGTGAG CAACCCGCAA ACGGTGGGGG TCGGCCGGGG CCGCTTCACC ACTTACGAAA 241 TCAGGGTCAA GACAAATCTT CCTATTTTCA AGCTGAAAGA ATCTACTGTT AGAAGAAGAT 301 ACAGTGACTT TGAATGGCTG CGAAGTGAAT TAGAAAGAGA GAGCAAGCCC TGCCTCAGAA 361 TGACATCAGA GGCAAGGAGT CATGGAAGGA CGTGGTGTGC TCAGAATGCT GAAAAGTTAT 421 TTTCTGACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTAGGT TACCGGTGGA CTGATAGCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt