Construct: ORF TRCN0000469226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015489.1_s317c1
- Derived from:
- ccsbBroadEn_13376
- DNA Barcode:
- GATCTCTTAAACCATCCATGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR90 (197335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 197335 | WDR90 | WD repeat domain 90 | XM_017023024.1 | 30.6% | 27.9% | (many diffs) |
| 2 | human | 197335 | WDR90 | WD repeat domain 90 | NM_145294.5 | 16.5% | 15.3% | (many diffs) |
| 3 | human | 197335 | WDR90 | WD repeat domain 90 | XM_017023023.1 | 15.8% | 14.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ctggtttgga cagcccggcc ctgggggctg gaataggaaa tgcctgcgtc 121 cctttaccct gcccaggggc caacaccccc agcctagcta cggcttcccc caggtgaacg 181 aggtggtctt cagccccggg gagtcccact gcgccacatg cagtgaggat gggagtgtgc 241 gggtgtgggc cttggccagc atggagcttg tgatccagtt ccaggtgctg aaccagagct 301 gcctctgcct ggcatggagc cccccgtgct gtggccgccc tgagcagcag cggctagcgg 361 ctggctacgg tgacggctcc ctgcgcatct tcagcgtctc ccgcacggcc atggagctca 421 agatgcaccc ccacccggtg gcgctgacca ctgttgcctt ctccaccgat ggtcagactg 481 tcctctctgg agacaaggat gggctcgtgg ctgtgagcca cccctgcaca gggacaaccT 541 TCCGTGTGCT GAGTGACCAC CAGGGCGCCC CAATCTCTAC CATCTGTGTC ACGTGCAAAG 601 AGACTCAGGG CCACCTGCCA CCCTCCCTCG CTGCCTTCTG CCCTTGGGAT GGGGCGCTCC 661 TGATGTACGT GGGCCCCGGT GTTTACAAGG AGGTGATCAT CTACAACCTC TGCCAGAAGC 721 AGGTGGTGGA GAAGATACCA CTGCCCTTTT TTGCCATGTC CCTGAGCCTG TCCCCCGGGA 781 CCCACCTCCT GGCTGTTGGC TTTGCTGAGT GCATGCTGAG GCTGGTAGAC TGTGCCATGG 841 GGACTGCCCA AGACTTTGCC GGCCACGACA ACGCAGTGCA CCTGTGCAGG TTTACACCGT 901 CCGCCAGGCT GCTCTTCACG GCCGCCCGCA ACGAGATCCT TGTGTGGGAG GTCCCCGGCC 961 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGAGA TCTCTTAAAC CATCCATGTA TCACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt