Construct: ORF TRCN0000469229
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016788.1_s317c1
- Derived from:
- ccsbBroadEn_03950
- DNA Barcode:
- ACTGCAGCGCCGCATAGACTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG3 (64422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469229
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64422 | ATG3 | autophagy related 3 | NM_022488.5 | 100% | 100% | |
| 2 | human | 64422 | ATG3 | autophagy related 3 | NM_001278712.2 | 93.4% | 93.3% | (many diffs) |
| 3 | human | 64422 | ATG3 | autophagy related 3 | XM_011513074.1 | 72.2% | 72.2% | 0_1ins261 |
| 4 | mouse | 67841 | Atg3 | autophagy related 3 | NM_026402.3 | 92.3% | 97.4% | (many diffs) |
| 5 | mouse | 67841 | Atg3 | autophagy related 3 | XM_006522495.3 | 66.4% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1008
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaatgtgatt aatactgtga agggaaaggc actggaagtg gctgagtacc 121 tgaccccggt cctcaaggaa tcaaagttta aggaaacagg tgtaattacc ccagaagagt 181 ttgtggcagc tggagatcac ctagtccacc actgtccaac atggcaatgg gctacagggg 241 aagaattgaa agtgaaggca tacctaccaa caggcaaaca atttttggta accaaaaatg 301 tgccgtgcta taagcggtgc aaacagatgg aatattcaga tgaattggaa gctatcattg 361 aagaagatga tggtgatggc ggatgggtag atacatatca caacacaggt attacaggaa 421 taacggaagc cgttaaagag atcacactgg aaaataagga caatataagg cttcaagatt 481 gctcagcact atgtgaagag gaagaagatg aagatgaagg agaagctgca gatatggaag 541 aatatgaaga gagtggattg ttggaaacag atgaggctac cctagataca aggaaaATAG 601 TAGAAGCTTG TAAAGCCAAA ACTGATGCTG GCGGTGAAGA TGCTATTTTG CAAACCAGAA 661 CTTATGACCT TTACATCACT TATGATAAAT ATTACCAGAC TCCACGATTA TGGTTGTTTG 721 GCTATGATGA GCAACGGCAG CCTTTAACAG TTGAGCACAT GTATGAAGAC ATCAGTCAGG 781 ATCATGTGAA GAAAACAGTG ACCATTGAAA ATCACCCTCA TCTGCCACCA CCTCCCATGT 841 GTTCAGTTCA CCCATGCAGG CATGCTGAGG TGATGAAGAA AATCATTGAG ACTGTTGCAG 901 AAGGAGGGGG AGAACTTGGA GTTCATATGT ATCTTCTTAT TTTCTTGAAA TTTGTACAAG 961 CTGTCATTCC AACAATAGAA TATGACTACA CAAGACACTT CACAATGTAC CCAACTTTCT 1021 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1081 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1141 GTGGAAAGGA CGAACTGCAG CGCCGCATAG ACTTTGAACG CGTTAAGTCg acaatcaacc 1201 tctggattac aaaatttgtg aaagatt