Construct: ORF TRCN0000469233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007746.1_s317c1
- Derived from:
- ccsbBroadEn_02236
- DNA Barcode:
- TAGCCGCCGTTACGCCTATTCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOX (9760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469233
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9760 | TOX | thymocyte selection associa... | NM_014729.3 | 100% | 100% | |
| 2 | human | 9760 | TOX | thymocyte selection associa... | XM_017014085.1 | 95.8% | 95.8% | 100_101ins66 |
| 3 | mouse | 252838 | Tox | thymocyte selection-associa... | NM_145711.4 | 89.1% | 94.2% | (many diffs) |
| 4 | mouse | 252838 | Tox | thymocyte selection-associa... | XM_006537944.3 | 89.1% | 94.2% | (many diffs) |
| 5 | mouse | 252838 | Tox | thymocyte selection-associa... | XM_006537945.2 | 89.1% | 94.2% | (many diffs) |
| 6 | mouse | 252838 | Tox | thymocyte selection-associa... | XM_017320230.1 | 82% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1644
- ORF length:
- 1578
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgtaagattt tatccacctc cagcccagcc cgccgctgcg cccgacgctc 121 cctgtctggg accttctccc tgcctggacc cctactattg caacaagttt gacggtgaga 181 acatgtatat gagcatgaca gagccgagcc aggactatgt gccagccagc cagtcctacc 241 ctggtccaag cctggaaagt gaagacttca acattccacc aattactcct ccttccctcc 301 cagaccactc gctggtgcac ctgaatgaag ttgagtctgg ttaccattct ctgtgtcacc 361 ccatgaacca taatggcctg ctaccatttc atccacaaaa catggacctc cctgaaatca 421 cagtctccaa tatgctgggc caggatggaa cactgctttc taattccatt tctgtgatgc 481 cagatatacg aaacccagaa ggaactcagt acagttccca tcctcagatg gcagccatga 541 gaccaagggg ccagcctgca gacatcaggc agcagccagg aatgatgcca catggccagc 601 tgactaccat taaccagtca cagctaagtg ctcaacttgg tttgaatatg ggaggaagca 661 atgttcccca caactcacca tctccacctg gaagcaagtc tgcaactcct tcaccatcca 721 gttcagtgca tgaagatgaa ggcgatgata cctctaagat caatggtgga gagaagcggc 781 ctgcctctga tatggggaaa aaaccaaaaa ctcccaaaaa gaagaagaag aaggatccca 841 atgagcccca gaagcctgtg tctgcctatg cgttattctt tcgtgatact caggccgcca 901 tcaagggcca aaatccaaac gctacctttg gcgaagtctc taaaattgtg gcttcaatgt 961 gggacggttt aggagaagag caaaaacagg tctataaaaa gaaaaccgag gctgcgaaga 1021 aggagtacct gaagcaactc gcagcataca gagccagcct tgtatccaag agctacagtg 1081 aacctgttga cgtgaagaca tctcaacctc ctcagctgat caattcgaag ccgtcggtgt 1141 tccatgggcc cagccaggcc cactcggccc tgtacctaag ttcccactat caccaacaac 1201 cgggaatgaa tcctcaccta actgccatgc atcctagtct ccccaggaac atagccccca 1261 agccgaataa ccaaatgcca gtgactgtct ctatagcaaa catggctgtg tccccTCCTC 1321 CTCCCCTCCA GATCAGCCCG CCTCTTCACC AGCATCTCAA CATGCAGCAG CACCAGCCGC 1381 TCACCATGCA GCAGCCCCTT GGGAACCAGC TCCCCATGCA GGTCCAGTCT GCCTTACACT 1441 CACCCACCAT GCAGCAAGGA TTTACTCTTC AACCCGACTA TCAGACTATT ATCAATCCTA 1501 CATCTACAGC TGCACAAGTT GTCACCCAGG CAATGGAGTA TGTGCGTTCG GGGTGCAGAA 1561 ATCCTCCCCC ACAACCGGTG GACTGGAATA ACGACTACTG CAGTAGTGGG GGCATGCAGA 1621 GGGACAAAGC ACTGTACCTT ACTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGCCGCCGTT 1801 ACGCCTATTC TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att