Transcript: Mouse XM_006537945.2

PREDICTED: Mus musculus thymocyte selection-associated high mobility group box (Tox), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tox (252838)
Length:
2773
CDS:
206..1786

Additional Resources:

NCBI RefSeq record:
XM_006537945.2
NBCI Gene record:
Tox (252838)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006537945.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000019477 CCCTACTATTGCAACAAGTTT pLKO.1 290 CDS 100% 5.625 7.875 N TOX n/a
2 TRCN0000019476 CGACTATCAGACTATTATCAA pLKO.1 1615 CDS 100% 5.625 7.875 N TOX n/a
3 TRCN0000084179 GCCAGAATCCAAATGCTACTT pLKO.1 1047 CDS 100% 4.950 3.960 N Tox n/a
4 TRCN0000428770 AGAACAGAAACAGGTCTATAA pLKO_005 1117 CDS 100% 13.200 9.240 N Tox n/a
5 TRCN0000423978 AGAAGAAGAAGGATCCTAATG pLKO_005 963 CDS 100% 10.800 7.560 N HMGB3 n/a
6 TRCN0000412871 AGAGGGACAAAGCGCTGTATC pLKO_005 1758 CDS 100% 10.800 7.560 N Tox n/a
7 TRCN0000419275 AGGATGCCTCCAAGATCAATG pLKO_005 885 CDS 100% 10.800 7.560 N Tox n/a
8 TRCN0000084182 GTCAACTCAAAGCCGTCAGTA pLKO.1 1259 CDS 100% 4.950 3.465 N Tox n/a
9 TRCN0000084181 AGGGCCAGAATCCAAATGCTA pLKO.1 1044 CDS 100% 3.000 2.100 N Tox n/a
10 TRCN0000084178 CCATTCTGATTTCTGTTGGTT pLKO.1 2015 3UTR 100% 3.000 2.100 N Tox n/a
11 TRCN0000084180 CTGTACCTAAGTTCTCACTAT pLKO.1 1310 CDS 100% 4.950 2.970 N Tox n/a
12 TRCN0000160007 CAAGAAGAAGAAGAAGAAGTA pLKO.1 955 CDS 100% 4.950 2.475 Y FAM98C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006537945.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02236 pDONR223 100% 89.1% 94.2% None (many diffs) n/a
2 ccsbBroad304_02236 pLX_304 0% 89.1% 94.2% V5 (many diffs) n/a
3 TRCN0000469233 TAGCCGCCGTTACGCCTATTCTCA pLX_317 21.2% 89.1% 94.2% V5 (many diffs) n/a
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