Construct: ORF TRCN0000469237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003880.1_s317c1
- Derived from:
- ccsbBroadEn_12803
- DNA Barcode:
- AATACCCTTACAGACCATGGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRXL2A (84293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469237
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84293 | PRXL2A | peroxiredoxin like 2A | NM_001243781.2 | 100% | 100% | |
| 2 | human | 84293 | PRXL2A | peroxiredoxin like 2A | NM_001243782.2 | 96.8% | 96.8% | 1_21del |
| 3 | human | 84293 | PRXL2A | peroxiredoxin like 2A | NM_001243778.1 | 95.1% | 95.1% | 1_33del |
| 4 | human | 84293 | PRXL2A | peroxiredoxin like 2A | NM_001243779.1 | 95.1% | 95.1% | 1_33del |
| 5 | human | 84293 | PRXL2A | peroxiredoxin like 2A | NM_001243780.1 | 95.1% | 95.1% | 1_33del |
| 6 | human | 84293 | PRXL2A | peroxiredoxin like 2A | NM_032333.5 | 95.1% | 95.1% | 1_33del |
| 7 | human | 84293 | PRXL2A | peroxiredoxin like 2A | XM_011540265.2 | 95.1% | 95.1% | 1_33del |
| 8 | human | 84293 | PRXL2A | peroxiredoxin like 2A | XM_011540266.2 | 95.1% | 95.1% | 1_33del |
| 9 | human | 84293 | PRXL2A | peroxiredoxin like 2A | XM_017016775.1 | 92.3% | 92.3% | 1_54del |
| 10 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316737.1 | 83.4% | 87.1% | (many diffs) |
| 11 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316739.1 | 83.4% | 87.1% | (many diffs) |
| 12 | mouse | 70564 | Fam213a | family with sequence simila... | NM_027464.4 | 83.4% | 87.1% | (many diffs) |
| 13 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316740.1 | 80.8% | 84.4% | (many diffs) |
| 14 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316734.1 | 79.4% | 82.9% | (many diffs) |
| 15 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316735.1 | 79.4% | 82.9% | (many diffs) |
| 16 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316736.1 | 79.4% | 82.9% | (many diffs) |
| 17 | mouse | 70564 | Fam213a | family with sequence simila... | XM_006519531.1 | 79.4% | 82.9% | (many diffs) |
| 18 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316732.1 | 78.4% | 81.8% | (many diffs) |
| 19 | mouse | 70564 | Fam213a | family with sequence simila... | NM_001316733.1 | 78.4% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gatgtggtcc attggtgcag gagccctggg ggctgctgcc ttggcattgc 121 tgcttgccaa cacagacgtg tttctgtcca agccccagaa agcggccctg gagtacctgg 181 aggatataga cctgaaaaca ctggagaagg aaccaaggac tttcaaagca aaggagctat 241 gggaaaaaaa tggagctgtg attatggccg tgcggaggcc aggctgtttc ctctgtcgag 301 aggaagctgc ggatctgtcc tcccTGAAAA GCATGTTGGA CCAGCTGGGC GTCCCCCTCT 361 ATGCAGTGGT AAAGGAGCAC ATCAGGACTG AAGTGAAGGA TTTCCAGCCT TATTTCAAAG 421 GAGAAATCTT CCTGGATGAA AAGAAAAAGT TCTATGGTCC ACAAAGGCGG AAGATGATGT 481 TTATGGGATT TATCCGTCTG GGAGTGTGGT ACAACTTCTT CCGAGCCTGG AACGGAGGCT 541 TCTCTGGAAA CCTGGAAGGA GAAGGCTTCA TCCTTGGGGG AGTTTTCGTG GTGGGATCAG 601 GAAAGCAGGG CATTCTTCTT GAGCACCGAG AAAAAGAATT TGGAGACAAA GTAAACCTAC 661 TTTCTGTTCT GGAAGCTGCT AAGATGATCA AACCACAGAC TTTGGCCTCA GAGAAAAAAT 721 GTCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGAAATAC CCTTACAGAC CATGGATACA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt