Construct: ORF TRCN0000469246
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016938.1_s317c1
- Derived from:
- ccsbBroadEn_13081
- DNA Barcode:
- GCCAAAGGGTCAGTCTTAGTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPH2 (121278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469246
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 121278 | TPH2 | tryptophan hydroxylase 2 | NM_173353.4 | 98.6% | 98.7% | 105_106insGCTTCGACATTCCTGAAG;936A>G;1125A>T |
| 2 | mouse | 216343 | Tph2 | tryptophan hydroxylase 2 | NM_173391.3 | 86.7% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1557
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagccagca atgatgatgt tttccagtaa atactgggca cggagagggt 121 tttccctgga ttcagcagtg cccgaagagc atcagctact tggcagctca acagcttcga 181 cattcctgaa gctaaataaa cctaactctg gcaaaaatga cgacaaaggc aacaagggaa 241 gcagcaaacg tgaagctgct accgaaagtg gcaagacagc agttgttttc tccttgaaga 301 atgaagttgg tggattggta aaagcactga ggctctttca ggaaaaacgt gtcaacatgg 361 ttcatattga atccaggaaa tctcggcgaa gaagttctga ggttgaaatc tttgtggact 421 gtgagtgtgg gaaaacagaa ttcaatgagc tcattcagtt gctgaaattt caaaccacta 481 ttgtgacgct gaatcctcca gagaacattt ggacagagga agaagagcta gaggatgtgc 541 cctggttccc tcggaagatc tctgagttag acaaatgctc tcacagagtt ctcatgtatg 601 gttctgagct tgatgctgac cacccaggat ttaaggacaa tgtctatcga cagagaagaa 661 agtattttgt ggatgtggcc atgggttata aatatggtca gcccattccc agggtggagt 721 atactgaaga agaaactaaa acttggggtg ttgtattccg ggagctctcc aaactctatc 781 ccactcatgc ttgccgagag tatttgaaaa acttccctct gctgactaaa tactgtggct 841 acagagagga caatgtgcct caactcgaag atgtctccat gtttctgaaa gaaaggtctg 901 gcttcacggt gaggccggtg gctggatacc tgagcccacg agactttctg gcaggactgg 961 cctacagagt gttccactgt acccagtaca tccggcatgg ctcagatccc ctctacaccc 1021 cggaaccaga cacatgccat gaactcttgg gacatgttcc actacttgcg gatcctaagt 1081 ttgctcagtt ttcacaagaa ataggtctgg cgtctctggg agcatcagat gaagatgttc 1141 agaaactagc cacgtgctat ttcttcacaa tcgagttTGG CCTTTGCAAG CAAGAAGGGC 1201 AACTGCGGGC TTATGGAGCA GGACTCCTTT CCTCCATTGG AGAATTAAAG CACGCCCTTT 1261 CTGACAAGGC ATGTGTGAAA GCCTTTGACC CAAAGACAAC TTGCTTACAG GAATGCCTTA 1321 TCACCACCTT CCAGGAAGCC TACTTTGTTT CAGAAAGTTT TGAAGAAGCC AAAGAAAAGA 1381 TGAGGGACTT TGCAAAGTCA ATTACCCGTC CCTTCTCAGT ATACTTCAAT CCCTACACAC 1441 AGAGTATTGA AATTCTGAAA GACACCAGAA GTATTGAAAA TGTGGTGCAG GACCTTCGCA 1501 GCGACTTGAA TACAGTGTGT GATGCTTTAA ACAAAATGAA CCAATATCTG GGGATTTTGC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GAGCCAAAGG GTCAGTCTTA GTGCCAACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt