Construct: ORF TRCN0000469247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012043.1_s317c1
- Derived from:
- ccsbBroadEn_13106
- DNA Barcode:
- ACCTCAGAATGCCGACACAAGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSF5 (124535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124535 | HSF5 | heat shock transcription fa... | NM_001080439.3 | 79.6% | 79.6% | 157_519del |
| 2 | human | 124535 | HSF5 | heat shock transcription fa... | XM_011524283.1 | 78.7% | 78.7% | 157_519del;1017_1037del |
| 3 | mouse | 327992 | Hsf5 | heat shock transcription fa... | NM_001045527.1 | 69% | 68.3% | (many diffs) |
| 4 | mouse | 327992 | Hsf5 | heat shock transcription fa... | XR_879708.2 | 37.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1494
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcgctg ctctccaccc ccatcaaccc caacaacttc cccgccaagc 121 tgtggcgcct ggtgaacagc ccgcgctacc gctccatccg ctgggacggc cgcggcgagg 181 ggctgcttat cgatcagccg ctcttcgagg ccgagctgct cagcccgccc gcggggcccc 241 ggcccgagcc gcacggacca gtggctgtag gacaatttca ccggtcattt cgtcgagata 301 gtttgtctcc ttactcctgt gtatcaactc catcccacga ccacagtact taccctctga 361 aaggtttaga tcggacccca gttcctcata gaatatggca gaactccctt ggaatgcatc 421 ctggacaagt ggagacatct cccacatttt cagataaagg ggttccgttt cctgtactcc 481 agaggtttcc aactgaggtt acctatacac tgcagcccag caccacatct gtacatgttc 541 aacaaggtcc tcaaacaatg gtcagctcct cccaaaaata cagtaactac acaccctcag 601 cacagtactc gcaagcctac tatccaacag ctgtgctaca gtgctgttct cctacccaca 661 tggatgctct aagtagttgt gtcactccca ctgcctcttc ctatgcacac tgcaactact 721 tccagaatcc ttcaatgcag tcctcctatc cagttgaatt tttgccttcc aattggccct 781 gcagtactac tgatgaaaat acaaagacag aagtaaacct agaggctgtc tttcagatag 841 ttgatgagtt gcattcctcc cctaaattgg agatggtaaa ggtggagcct gttgagaatc 901 agtgcccaac atctccgtct tacagaggcc aacacatttt agctaattct aacaacagca 961 atccatgttc tgcaagtcag gctagccagc tggagccact tactcctgta ggctcagata 1021 ttatgtcttt tgtggttgga aCAGAACAAG CAGTTGCCTG CTCTCTACCA CAGTCACCTG 1081 AGTACATCTA TACCATCCAC ACAGCTCAGC CTGTTGAAAA TAGCACAATA CAGGAATCTG 1141 CAGCCATCCA GCAAGCTCAT GTCAAACTGA AGGAGCACCT AAATCATAAT CCATCTCCAT 1201 CTTCAGTAGT ATTTGTGCAG GAAGGGCCAC CATTCAGCAC ACACCAGGTG GATGCCAACA 1261 TAAAATGCCA GACCAGTTCA CGTGAGAATA TCTTGCCGTC AGAACAGATG GGATTCCTCA 1321 TTTCAGAAAT GGGGCCTGCT AGCAAGCCTA GTGAAGACAC AGGTTTAGCC ACTCCAGCCA 1381 GATACAGAGA GCACAGAAGC AACTCACAAC AAGGAAAGTC CCCTGATCTT CATCTGCTGG 1441 TGGACGTGGC CTGCAAGCAG GAGCGCTTTC CAAAGGAGGA AGAATTAAAA GAGTTGCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAA CCTCAGAATG CCGACACAAG TCCACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att