Construct: ORF TRCN0000469249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001891.1_s317c1
- Derived from:
- ccsbBroadEn_09715
- DNA Barcode:
- TAAAAATGACTCATACCGGTTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC7A13 (157724)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157724 | SLC7A13 | solute carrier family 7 mem... | NM_138817.3 | 99.9% | 99.7% | 745G>A |
| 2 | human | 157724 | SLC7A13 | solute carrier family 7 mem... | XM_011516867.2 | 98% | 97.8% | 655_656ins27;718G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1479
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatagaggg gagaaaatac agctcaagag agtgtttgga tattggtggg 121 gcacaagttt tttgcttatt aatatcattg gtgcaggaat ttttgtgtcc cccaaaggtg 181 tgttggcata ctcttgcatg aacgtgggag tctccctgtg cgtttgggct ggctgtgcca 241 tactggccat gacatcaact ctttgctctg cagagataag tataagcttc ccatgcagtg 301 gagctcaata ctattttctc aagagatact ttggctccac ggttgctttt ttgaatctct 361 ggacatcctt gtttctgggg tcaggggtag ttgctggcca agctctgctc cttgctgagt 421 acagcatcca gccttttttt cccagctgct ctgtcccaaa gctgcctaag aaatgtctgg 481 cattggccat gttgtggatt gtaggaattc tgacttctcg tggtgtgaaa gaagtgactt 541 ggcttcagat agctagctca gtgctgaaag tgtccatact tagcttcatt tccctaactg 601 gagtagtgtt cctgataaga gggaaaaagg agaatgtaga acgatttcag aatgcttttg 661 atgctgaact tccagatatc tctcacctta tacaagccat cttccaagga tattttgcat 721 attcaggcgg ggcatgcttt acacttatag caggggagct gaagaagccc agaacaacaa 781 ttcccaaatg catatttact gcgttacctc tgatgactgt agtttattta ctggttaaca 841 tttcctatct gactgttctg acacccaggg aaattctctc ttcagatgct gtagctatca 901 catgggctga tcgagctttt ccctcattag catggattat gccttttgct atttctacct 961 cattatttag caaccttctg atttctatat ttaaatcatc gagaccaata tatcttgcaa 1021 gccaagaggg CCAGCTGCCT TTGCTATTTA ATACACTTAA TAGTCACTCT TCTCCATTTA 1081 CAGCTGTGCT ACTACTTGTC ACTTTGGGAT CCCTTGCAAT TATCTTAACA AGTCTAATTG 1141 ATTTGATAAA CTATATTTTT TTCACGGGTT CATTATGGTC TATATTATTA ATGATAGGAA 1201 TACTAAGGCG GAGATACCAG GAACCCAATC TATCTATACC TTATAAGGTG TTTTTGTCAT 1261 TTCCATTAGC AACAATAGTC ATCGACGTGG GCTTGGTTGT GATACCATTG GTAAAGTCTC 1321 CAAATGTGCA TTATGTCTAC GTGCTTCTGT TAGTTCTCAG CGGATTACTA TTTTACATAC 1381 CTTTAATACA TTTTAAAATA AGATTGGCTT GGTTTGAGAA GATGACTTGC TATTTACAAT 1441 TACTATTTAA TATTTGCCTC CCTGATGTGT CTGAGGAATT GCCAACTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGATAAAAA TGACTCATAC CGGTTAGGAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt