Construct: ORF TRCN0000469257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011134.1_s317c1
- Derived from:
- ccsbBroadEn_01546
- DNA Barcode:
- AAGTTTCTCATTACTCGCTGTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC7A1 (6541)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6541 | SLC7A1 | solute carrier family 7 mem... | NM_003045.5 | 99.8% | 100% | 1401C>G;1506C>T |
| 2 | human | 6541 | SLC7A1 | solute carrier family 7 mem... | XM_005266507.3 | 99.8% | 100% | 1401C>G;1506C>T |
| 3 | human | 6541 | SLC7A1 | solute carrier family 7 mem... | XM_017020713.2 | 95.1% | 86.6% | (many diffs) |
| 4 | mouse | 11987 | Slc7a1 | solute carrier family 7 (ca... | NM_001301424.1 | 83.8% | 86.6% | (many diffs) |
| 5 | mouse | 11987 | Slc7a1 | solute carrier family 7 (ca... | NM_007513.4 | 83.8% | 86.6% | (many diffs) |
| 6 | mouse | 11987 | Slc7a1 | solute carrier family 7 (ca... | XM_006504796.1 | 83.8% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1953
- ORF length:
- 1887
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtgcaaagtc ctgctcaaca ttgggcagca gatgctgcgg cggaaggtgg 121 tggactgtag ccgggaggag acgcggctgt ctcgctgcct gaacactttt gatctggtgg 181 ccctcggggt gggcagcaca ctgggtgctg gtgtctacgt cctggctgga gctgtggccc 241 gtgagaatgc aggccctgcc attgtcatct ccttcctgat cgctgcgctg gcctcagtgc 301 tggctggcct gtgctatggc gagtttggtg ctcgggtccc caagacgggc tcagcttacc 361 tctacagcta tgtcaccgtt ggagagctct gggccttcat caccggctgg aacttaatcc 421 tctcctacat catcggtact tcaagcgtag cgagggcctg gagcgccacc ttcgacgagc 481 tgataggcag acccatcggg gagttctcac ggacacacat gactctgaac gcccccggcg 541 tgctggctga aaaccccgac atattcgcag tgatcataat tctcatcttg acaggacttt 601 taactcttgg tgtgaaagag tcggccatgg tcaacaaaat attcacttgt attaacgtcc 661 tggtcctggg cttcataatg gtgtcaggat ttgtgaaagg atcggttaaa aactggcagc 721 tcacggagga ggattttggg aacacatcag gccgtctctg tttgaacaat gacacaaaag 781 aagggaagcc cggtgttggt ggattcatgc ccttcgggtt ctctggtgtc ctgtcggggg 841 cagcgacttg cttctatgcc ttcgtgggct ttgactgcat cgccaccaca ggtgaagagg 901 tgaagaaccc acagaaggcc atccccgtgg ggatcgtggc gtccctcttg atctgcttca 961 tcgcctactt tggggtgtcg gctgccctca cgctcatgat gccctacttc tgcctggaca 1021 ataacagccc cctgcccgac gcctttaagc acgtgggctg ggaaggtgcc aagtacgcag 1081 tggccgtggg ctccctctgc gctctttccg ccagtcttct aggttccatg tttcccatgc 1141 ctcgggttat ctatgccatg gctgaggatg gactgctatt taaattctta gccaacgtca 1201 atgataggac caaaacacca ataatcgcca cattagcctc gggtgccgtt gctgctgtga 1261 tggccttcct ctttgacctg aaggacttgg tggacctcat gtccattggc actctcctgg 1321 cttactcgtt ggtggctgcc tgtgtgttgg tcttacggta ccagccagag cagcctaacc 1381 tggtatacca gatggccagt acttccgacg agttagatcc agcagaccaa aatgaattgg 1441 caagcaccaa tgattcccag ctggggtttt taccagaggc agagatgttc tctttgaaaa 1501 ccatactctc acccaaaaac atggagcctt ccaaaatctc tgggctaatt gtgaacattt 1561 caaccagccT TATAGCTGTT CTCATCATCA CCTTCTGCAT TGTGACCGTG CTTGGAAGGG 1621 AGGCTCTCAC CAAAGGGGCG CTGTGGGCAG TCTTTCTGCT CGCAGGGTCT GCCCTCCTCT 1681 GTGCCGTGGT CACGGGCGTC ATCTGGAGGC AGCCCGAGAG CAAGACCAAG CTCTCATTTA 1741 AGGTTCCCTT CCTGCCAGTG CTCCCCATCC TGAGCATCTT CGTGAACGTC TATCTCATGA 1801 TGCAGCTGGA CCAGGGCACC TGGGTCCGGT TTGCTGTGTG GATGCTGATA GGCTTCATCA 1861 TCTACTTTGG CTATGGCCTG TGGCACAGCG AGGAGGCGTC CCTGGATGCC GACCAAGCAA 1921 GGACTCCTGA CGGCAACTTG GACCAGTGCA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1981 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2041 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 2101 GTTTCTCATT ACTCGCTGTC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2161 ttgtgaaaga tt