Construct: ORF TRCN0000469263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017614.1_s317c1
- Derived from:
- ccsbBroadEn_08411
- DNA Barcode:
- CAGGATGACTGCGGTTACCCACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GIPC2 (54810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54810 | GIPC2 | GIPC PDZ domain containing ... | NM_017655.6 | 99.8% | 99.6% | 617T>C |
| 2 | human | 54810 | GIPC2 | GIPC PDZ domain containing ... | NM_001304725.2 | 75.9% | 74.2% | (many diffs) |
| 3 | mouse | 54120 | Gipc2 | GIPC PDZ domain containing ... | XM_011240165.1 | 57.8% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cctgaagctg cgggggaaga agaaggccaa gtccaaggag accgccgggc 121 tggtggaggg cgagccgacg ggcgcgggcg gcgggagcct ctcagcgtcc cgggctcccg 181 cacgcaggct ggtcttccac gcgcagctgg cgcacggtag tgccacgggc cgagtggagg 241 gcttctccag catccaggag ctctacgccc agatcgcggg cgcgtttgaa atctcgccgt 301 cggagatctt atattgcact ttaaacacac ctaaaattga catggaaaga ctcttaggag 361 gacaactagg actagaagat ttcatatttg cccatgtgaa aggaatcgaa aaagaagtga 421 atgtgtataa atctgaggat tcacttggtc tcaccattac agataatggt gttggctatg 481 cttttataaa gagaattaaa gatggtggtg ttattgactc agttaaaaca atctgtgttg 541 gggatcatat tgaatccata aatggagaaa atattgttgg gtggcgtcac tatgatgttg 601 ctaagaagtt aaaggaatta aaaaaggagg aactctttac tatgaagtta atagaaccta 661 agaaggcatt tGAAATAGAG CCGAGGTCAA AGGCTGGAAA GTCATCAGGA GAAAAAATTG 721 GTTGTGGAAG GGCAACACTT CGCCTGAGAT CAAAAGGTCC TGCCACCGTG GAAGAAATGC 781 CTTCTGAAAC CAAAGCAAAG GCAATTGAAA AGATTGATGA TGTTCTTGAG TTGTACATGG 841 GAATTCGAGA TATTGATTTA GCCACCACAA TGTTTGAAGC TGGAAAGGAC AAAGTAAATC 901 CAGATGAATT TGCTGTGGCA CTTGACGAAA CTCTTGGAGA CTTTGCGTTC CCAGACGAAT 961 TTGTCTTTGA TGTTTGGGGA GTCATTGGTG ATGCCAAACG AAGAGGATTA TGCCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGACAGG ATGACTGCGG TTACCCACAG ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt