Construct: ORF TRCN0000469265
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001184.1_s317c1
- Derived from:
- ccsbBroadEn_03327
- DNA Barcode:
- CGACTAACGGGTATCTCCCGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSKIP (51527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469265
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51527 | GSKIP | GSK3B interacting protein | NM_001271904.1 | 100% | 100% | |
| 2 | human | 51527 | GSKIP | GSK3B interacting protein | NM_001271905.1 | 100% | 100% | |
| 3 | human | 51527 | GSKIP | GSK3B interacting protein | NM_001271906.1 | 100% | 100% | |
| 4 | human | 51527 | GSKIP | GSK3B interacting protein | NM_016472.5 | 100% | 100% | |
| 5 | mouse | 66787 | Gskip | GSK3B interacting protein | NM_178613.3 | 84.7% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aacagactgt aatcccatgg agctaagcag tatgtcagga tttgaagaag 121 gttcagagct gaacggtttt GAAGGAACTG ACATGAAAGA CATGAGGCTC GAAGCTGAAG 181 CAGTTGTAAA TGATGTTCTC TTTGCTGTTA ACAACATGTT TGTCTCGAAA AGCCTGCGGT 241 GTGCGGATGA TGTGGCCTAT ATCAATGTGG AAACAAAGGA AAGAAACAGA TATTGCCTAG 301 AACTCACTGA AGCAGGGCTC AAGGTGGTAG GCTATGCTTT TGACCAGGTA GATGATCATT 361 TACAGACTCC CTACCATGAA ACAGTCTACT CCTTGTTGGA TACACTCAGC CCCGCCTACC 421 GAGAAGCATT TGGAAACGCA CTGCTTCAAA GACTGGAAGC TTTGAAAAGA GATGGACAGT 481 CATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGACG ACTAACGGGT ATCTCCCGGT TGACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt