Construct: ORF TRCN0000469280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002542.1_s317c1
- Derived from:
- ccsbBroadEn_04081
- DNA Barcode:
- TCCTCTACACAGTTGTTGGGCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC73 (79577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79577 | CDC73 | cell division cycle 73 | NM_024529.4 | 100% | 100% | |
| 2 | human | 79577 | CDC73 | cell division cycle 73 | XM_006711537.4 | 63.8% | 61.2% | (many diffs) |
| 3 | mouse | 214498 | Cdc73 | cell division cycle 73, Paf... | NM_145991.2 | 92.6% | 100% | (many diffs) |
| 4 | mouse | 214498 | Cdc73 | cell division cycle 73, Paf... | XM_006529365.1 | 58.7% | 58.6% | (many diffs) |
| 5 | mouse | 214498 | Cdc73 | cell division cycle 73, Paf... | XM_006529366.3 | 56.9% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgtgctt agcgtcctgc gacagtacaa catccagaag aaggagattg 121 tggtgaaggg agacgaagtg atcttcgggg agttctcctg gcccaagaat gtgaagacca 181 actatgttgt ttgggggact ggaaaggaag gccaacccag agagtactac acattggatt 241 ccattttatt tctacttaat aacgtgcacc tttctcatcc tgtttatgtc cgacgtgcag 301 ctactgaaaa tattcctgtg gttagaagac ctgatcgaaa agatctactt ggatatctca 361 atggtgaagc gtcaacatcg gcaagtatag acagaagcgc tcccttagaa ataggtcttc 421 agcgatctac tcaagtcaaa cgagctgcag atgaagtttt agcagaagca aagaaaccac 481 gaattgagga tgaagagtgt gtgcgccttg ataaagagag attggctgcc cgtttggagg 541 gtcacaaaga agggattgta cagactgaac agattaggtc tttgtctgaa gctatgtcag 601 tggaaaaaat tgctgcaatc aaagccaaaa ttatggctaa gaaaagatct actatcaaga 661 ctgatctaga tgatgacata actgccctta aacagaggag ttttgtggat gctgaggtag 721 atgtgacccg agatattgtc agcagagaga gagtatggag gacacgaaca actatcttac 781 aaagcacagg aaagaatttt tccaagaaca tttttgcaat tcttcaatct gtaaaagcca 841 gagaagaagg gcgtgcacct gaacagcgac ctgccccaaa tgcagcacct gtggatccca 901 ctttgcgcac caaacagcct atcccagctg cctataacag atacgatcag gaaagattca 961 aaggaaaaga agaaacggaa ggcttcaaaa ttgacactat gggaacctac catggtatga 1021 cactgaaatc tgtaacggag ggtgcatctg cccggaagac tcagactcct gcagcccagc 1081 cagtaccaag accagtttct caagcaagac ctcccccaaa tcagaagaaa ggatctcgaa 1141 cacccattat cataattcct gcagctacca cctctttaat aaccatgctt aatgcaaaag 1201 accttctaca ggacctgaaa tttgtcccat cagatgaaaa gaagaaacaa ggttgtcaac 1261 gagaaaatga aactctaata caaagaagaa aagaccagat gcaaccaggg ggcactgcaa 1321 ttagtgttac agtaccttat agagtagtag accagcccct taaacttatg cctcaagact 1381 gggaccgcgt tgtagccgtt tttgtgcagg gtcctgcatg gcagttcaaa ggttgGCCAT 1441 GGCTTTTGCC TGATGGATCA CCAGTTGATA TATTTGCTAA AATTAAAGCC TTCCATCTGA 1501 AGTATGATGA AGTTCGTCTG GATCCAAATG TTCAGAAATG GGATGTAACA GTATTAGAAC 1561 TCAGCTATCA CAAACGTCAT TTGGATAGAC CAGTGTTCTT ACGGTTTTGG GAAACATTGG 1621 ACAGGTACAT GGTAAAGCAT AAATCGCACT TGAGATTCTG CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGATCCTCT ACACAGTTGT TGGGCCGTAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt