Construct: ORF TRCN0000469296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002574.1_s317c1
- Derived from:
- ccsbBroadEn_04166
- DNA Barcode:
- AGACCAATTCCTCTCTACAACCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBTD1 (80019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80019 | UBTD1 | ubiquitin domain containing 1 | NM_024954.5 | 100% | 100% | |
| 2 | mouse | 226122 | Ubtd1 | ubiquitin domain containing 1 | NM_145500.3 | 90.9% | 98.6% | (many diffs) |
| 3 | mouse | 226122 | Ubtd1 | ubiquitin domain containing 1 | XM_006526997.1 | 74% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caactgcgtg gggagacagc gccgggagag gccggcagcc ccgggacacc 121 cccgcaagcg agcaggacgc aatgagcccc tgaagaaaga gcggcttaag tggaagagcg 181 actaccccat gactgacggg cagctgcgga gcaaacggga tgagttctgg gacacagcgc 241 ctgccttcga gggccgcaag gagatctggg atgccctcaa ggctgccgcc tatgctgctg 301 aagccaacga ccacgagctg gccCAGGCCA TCCTGGATGG AGCCAGCATC ACCCTGCCTC 361 ATGGCACCCT CTGTGAATGC TACGATGAGC TGGGCAATCG CTACCAGCTG CCCATCTACT 421 GCCTGTCACC GCCGGTGAAC CTGCTGCTGG AGCACACGGA GGAGGAGAGC CTGGAGCCCC 481 CCGAGCCTCC ACCCAGCGTG CGCCGTGAGT TCCCGCTGAA GGTGCGCCTG TCCACGGGCA 541 AGGACGTGAG GCTCAGCGCC AGCCTGCCCG ACACAGTGGG GCAGCTCAAG AGGCAGCTGC 601 ACGCCCAGGA GGGCATCGAG CCATCGTGGC AGCGGTGGTT CTTCTCCGGG AAGCTGCTCA 661 CAGACCGCAC ACGGCTCCAG GAGACCAAGA TCCAGAAAGA TTTTGTCATC CAGGTCATCA 721 TCAACCAGCC CCCACCACCC CAGGACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGACCAAT 901 TCCTCTCTAC AACCCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt