Construct: ORF TRCN0000469311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008107.1_s317c1
- Derived from:
- ccsbBroadEn_05215
- DNA Barcode:
- GCCGTGCCGTAAACGGACTCCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAGE2 (203569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203569 | PAGE2 | PAGE family member 2 | NM_207339.4 | 99.6% | 100% | 279T>G |
| 2 | human | 203569 | PAGE2 | PAGE family member 2 | XM_017029353.1 | 99.6% | 100% | 279T>G |
| 3 | human | 389860 | PAGE2B | PAGE family member 2B | NM_001015038.3 | 96.3% | 93.6% | (many diffs) |
| 4 | human | 389860 | PAGE2B | PAGE family member 2B | XM_011530787.2 | 96.3% | 93.6% | (many diffs) |
| 5 | human | 389860 | PAGE2B | PAGE family member 2B | XM_017029513.1 | 96.3% | 93.6% | (many diffs) |
| 6 | human | 389860 | PAGE2B | PAGE family member 2B | XM_011530786.3 | 92.2% | 89.6% | (many diffs) |
| 7 | human | 90737 | PAGE5 | PAGE family member 5 | NM_001013435.3 | 92.1% | 81.9% | (many diffs) |
| 8 | human | 389860 | PAGE2B | PAGE family member 2B | XM_011530785.2 | 79.8% | 77.6% | (many diffs) |
| 9 | human | 90737 | PAGE5 | PAGE family member 5 | NM_130467.5 | 78.1% | 69.4% | (many diffs) |
| 10 | human | 100421746 | LOC100421746 | PAGE family member 5 pseudo... | NR_130179.1 | 59.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 423
- ORF length:
- 333
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGTGAGCT TCTAAGAGCA AGATCCCAAT 121 CCTCAGAAAG AGGAAATGAC CAAGAGTCTT CCCAGCCGGT TGGATCTGTG ATTGTCCAGG 181 AGCCCACTGA GGAAAAACGT CAAGAAGAGG AACCACCAAC TGATAATCAG GGTATTGCAC 241 CTAGTGGGGA GATTGAAAAT CAAGCAGTGC CTGCTTTTCA AGGGCCTGAC ATGGAAGCTT 301 TTCAACAGGA ACTGGCTCTG CTTAAGATAG AGGATGAGCC TGGAGATGGT CCTGATGTCA 361 GGGAGGGGAT TATGCCCACT TTTGATCTCA CTAAAGTGCT GGAAGCAGGT GATGCGCAAC 421 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGAGC CGTGCCGTAA ACGGACTCCA TCACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt